2hqa: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="2hqa" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hqa, resolution 2.600Å" /> '''Crystal structure o... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2hqa.gif|left|200px]]<br /><applet load="2hqa" size=" | [[Image:2hqa.gif|left|200px]]<br /><applet load="2hqa" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2hqa, resolution 2.600Å" /> | caption="2hqa, resolution 2.600Å" /> | ||
'''Crystal structure of the catalytic alpha subunit of E. Coli replicative DNA polymerase III'''<br /> | '''Crystal structure of the catalytic alpha subunit of E. Coli replicative DNA polymerase III'''<br /> | ||
==Overview== | ==Overview== | ||
Bacterial replicative DNA polymerases such as Polymerase III (Pol III) | Bacterial replicative DNA polymerases such as Polymerase III (Pol III) share no sequence similarity with other polymerases. The crystal structure, determined at 2.3 A resolution, of a large fragment of Pol III (residues 1-917), reveals a unique chain fold with localized similarity in the catalytic domain to DNA polymerase beta and related nucleotidyltransferases. The structure of Pol III is strikingly different from those of members of the canonical DNA polymerase families, which include eukaryotic replicative polymerases, suggesting that the DNA replication machinery in bacteria arose independently. A structural element near the active site in Pol III that is not present in nucleotidyltransferases but which resembles an element at the active sites of some canonical DNA polymerases suggests that, at a more distant level, all DNA polymerases may share a common ancestor. The structure also suggests a model for interaction of Pol III with the sliding clamp and DNA. | ||
==About this Structure== | ==About this Structure== | ||
2HQA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http:// | 2HQA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HQA OCA]. | ||
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Donnell, M | [[Category: Donnell, M O.]] | ||
[[Category: Georgescu, R | [[Category: Georgescu, R E.]] | ||
[[Category: Kuriyan, J.]] | [[Category: Kuriyan, J.]] | ||
[[Category: Lamers, M | [[Category: Lamers, M H.]] | ||
[[Category: Lee, S | [[Category: Lee, S G.]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
[[Category: dna polymerase iii]] | [[Category: dna polymerase iii]] | ||
| Line 26: | Line 26: | ||
[[Category: pol beta]] | [[Category: pol beta]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:44:31 2008'' | ||
