3p1y: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
{{STRUCTURE_3p1y| PDB=3p1y | SCENE= }} | {{STRUCTURE_3p1y| PDB=3p1y | SCENE= }} | ||
===Crystal structure of the chimeric Archaeoglobus fulgidus RNA splicing endonuclease with the broadest substrate specificity=== | ===Crystal structure of the chimeric Archaeoglobus fulgidus RNA splicing endonuclease with the broadest substrate specificity=== | ||
{{ABSTRACT_PUBMED_21846775}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/ENDA_ARCFU ENDA_ARCFU]] Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp. | |||
==About this Structure== | ==About this Structure== | ||
[[3p1y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1Y OCA]. | [[3p1y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeropyrum_pernix Aeropyrum pernix]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P1Y OCA]. | ||
== | ==Reference== | ||
<ref group="xtra">PMID:021846775</ref><references group="xtra"/><references/> | |||
[[Category: Aeropyrum pernix]] | [[Category: Aeropyrum pernix]] | ||
[[Category: TRNA-intron endonuclease]] | [[Category: TRNA-intron endonuclease]] | ||
Revision as of 07:32, 30 June 2013
Crystal structure of the chimeric Archaeoglobus fulgidus RNA splicing endonuclease with the broadest substrate specificity
Template:ABSTRACT PUBMED 21846775
Function
[ENDA_ARCFU] Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
About this Structure
3p1y is a 4 chain structure with sequence from Aeropyrum pernix. Full crystallographic information is available from OCA.
Reference
- Hirata A, Kitajima T, Hori H. Cleavage of intron from the standard or non-standard position of the precursor tRNA by the splicing endonuclease of Aeropyrum pernix, a hyper-thermophilic Crenarchaeon, involves a novel RNA recognition site in the Crenarchaea specific loop. Nucleic Acids Res. 2011 Nov;39(21):9376-89. Epub 2011 Aug 16. PMID:21846775 doi:10.1093/nar/gkr615
