2i7z: Difference between revisions

Line 4:

==Overview==

Metal ions are critical for the proper folding of RNA, and the GAAA, tetraloop-receptor is necessary for the optimal folding and function of, many RNAs. We have used NMR to investigate the role of metal ions in the, structure of the tetraloop-receptor in solution. The NMR data indicate, native tertiary structure is formed under a wide range of ionic, conditions. The lack of conformational adaptation in response to very, different ionic conditions argues against a structural role for divalent, ions. Nuclear Overhauser effects to cobalt hexammine and paramagnetic, relaxation enhancement induced by manganese ions were used to determine, the NMR structures of the tetraloop receptor in association with metal, ions, providing the first atomic-level view of these interactions in the, solution state. Five manganese and two cobalt hexammine ions could be, localized to the RNA surface. The locations of the associated metal ions, are similar, but not identical to, those of previously determined crystal, structures. The sites of association are in general agreement with, nonlinear Poisson-Boltzmann calculations of the electrostatic surface, emphasizing the general importance of diffusely associated ions in RNA, tertiary structure.

Metal ions are critical for the proper folding of RNA, and the GAAA tetraloop-receptor is necessary for the optimal folding and function of many RNAs. We have used NMR to investigate the role of metal ions in the structure of the tetraloop-receptor in solution. The NMR data indicate native tertiary structure is formed under a wide range of ionic conditions. The lack of conformational adaptation in response to very different ionic conditions argues against a structural role for divalent ions. Nuclear Overhauser effects to cobalt hexammine and paramagnetic relaxation enhancement induced by manganese ions were used to determine the NMR structures of the tetraloop receptor in association with metal ions, providing the first atomic-level view of these interactions in the solution state. Five manganese and two cobalt hexammine ions could be localized to the RNA surface. The locations of the associated metal ions are similar, but not identical to, those of previously determined crystal structures. The sites of association are in general agreement with nonlinear Poisson-Boltzmann calculations of the electrostatic surface, emphasizing the general importance of diffusely associated ions in RNA tertiary structure.

==About this Structure==

Line 12:

Role of metal ions in the tetraloop-receptor complex as analyzed by NMR., Davis JH, Foster TR, Tonelli M, Butcher SE, RNA. 2007 Jan;13(1):76-86. Epub 2006 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17119098 17119098]

[[Category: Protein complex]]

[[Category: Butcher, S.E.]]

[[Category: Butcher, S E.]]

[[Category: Davis, J.H.]]

[[Category: Davis, J H.]]

[[Category: MN6]]

[[Category: 11-nucleotide receptor]]

Line 19:

[[Category: rna tertiary structure]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jan 29 20~~:36:38 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:50:02 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-Metal ions are critical for the proper folding of RNA, and the GAAA, tetraloop-receptor is necessary for the optimal folding and function of, many RNAs. We have used NMR to investigate the role of metal ions in the, structure of the tetraloop-receptor in solution. The NMR data indicate, native tertiary structure is formed under a wide range of ionic, conditions. The lack of conformational adaptation in response to very, different ionic conditions argues against a structural role for divalent, ions. Nuclear Overhauser effects to cobalt hexammine and paramagnetic, relaxation enhancement induced by manganese ions were used to determine, the NMR structures of the tetraloop receptor in association with metal, ions, providing the first atomic-level view of these interactions in the, solution state. Five manganese and two cobalt hexammine ions could be, localized to the RNA surface. The locations of the associated metal ions, are similar, but not identical to, those of previously determined crystal, structures. The sites of association are in general agreement with, nonlinear Poisson-Boltzmann calculations of the electrostatic surface, emphasizing the general importance of diffusely associated ions in RNA, tertiary structure.
+Metal ions are critical for the proper folding of RNA, and the GAAA tetraloop-receptor is necessary for the optimal folding and function of many RNAs. We have used NMR to investigate the role of metal ions in the structure of the tetraloop-receptor in solution. The NMR data indicate native tertiary structure is formed under a wide range of ionic conditions. The lack of conformational adaptation in response to very different ionic conditions argues against a structural role for divalent ions. Nuclear Overhauser effects to cobalt hexammine and paramagnetic relaxation enhancement induced by manganese ions were used to determine the NMR structures of the tetraloop receptor in association with metal ions, providing the first atomic-level view of these interactions in the solution state. Five manganese and two cobalt hexammine ions could be localized to the RNA surface. The locations of the associated metal ions are similar, but not identical to, those of previously determined crystal structures. The sites of association are in general agreement with nonlinear Poisson-Boltzmann calculations of the electrostatic surface, emphasizing the general importance of diffusely associated ions in RNA tertiary structure.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 Role of metal ions in the tetraloop-receptor complex as analyzed by NMR., Davis JH, Foster TR, Tonelli M, Butcher SE, RNA. 2007 Jan;13(1):76-86. Epub 2006 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17119098 17119098]
 [[Category: Protein complex]]
-[[Category: Butcher, S.E.]]
+[[Category: Butcher, S E.]]
-[[Category: Davis, J.H.]]
+[[Category: Davis, J H.]]
 [[Category: MN6]]
 [[Category: 11-nucleotide receptor]]
@@ Line 19: / Line 19: @@
 [[Category: rna tertiary structure]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:36:38 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:50:02 2008''