2ifu: Difference between revisions

Soluble N-ethylmaleimide-sensitive factor attachment protein gamma, (gamma-SNAP) is a member of an eukaryotic protein family involved in, intracellular membrane trafficking. The X-ray structure of Brachydanio, rerio gamma-SNAP was determined to 2.6 A and revealed an all-helical, protein comprised of an extended twisted-sheet of helical hairpins with a, helical-bundle domain on its carboxy-terminal end. Structural and, conformational differences between multiple observed gamma-SNAP molecules, and Sec17, a SNAP family protein from yeast, are analyzed. Conformational, variation in gamma-SNAP molecules is matched with great precision by the, two lowest frequency normal modes of the structure. Comparison of the, lowest-frequency modes from gamma-SNAP and Sec17 indicated that the, structures share preferred directions of flexibility, corresponding to, bending and twisting of the twisted sheet motif. We discuss possible, consequences related to the flexibility of the SNAP proteins for the, mechanism of the 20S complex disassembly during the SNAP receptors, recycling.

2IFU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Danio_rerio Danio rerio] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:So4 Binding Site For Residue A 306'>AC1</scene>, <scene name='pdbsite=AC2:So4 Binding Site For Residue A 307'>AC2</scene>, <scene name='pdbsite=AC3:So4 Binding Site For Residue A 308'>AC3</scene>, <scene name='pdbsite=AC4:So4 Binding Site For Residue B 306'>AC4</scene>, <scene name='pdbsite=AC5:So4 Binding Site For Residue B 307'>AC5</scene>, <scene name='pdbsite=AC6:So4 Binding Site For Residue B 308'>AC6</scene>, <scene name='pdbsite=AC7:So4 Binding Site For Residue B 309'>AC7</scene>, <scene name='pdbsite=AC8:So4 Binding Site For Residue C 306'>AC8</scene>, <scene name='pdbsite=AC9:So4 Binding Site For Residue C 307'>AC9</scene> and <scene name='pdbsite=BC1:So4 Binding Site For Residue D 306'>BC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFU OCA].  

[[Category: Bingman, C.A.]]

[[Category: CESG, Center.for.Eukaryotic.Structural.Genomics.]]

[[Category: Jr., G.N.Phillips.]]

[[Category: Mccoy, J.G.]]

[[Category: Wesenberg, G.E.]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 10:38:22 2008''