G14secL04Tpc3: Difference between revisions
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==Free OspB structure== | ==Free OspB structure== | ||
<Structure load='1p4p' size='350' frame='true' align='right' caption='Free OspB' scene = 'G14secL04Tpc3/Free_ospb_colored/2'/> | <Structure load='1p4p' size='350' frame='true' align='right' caption='Free OspB (PDB code [[1p4p]])' scene = 'G14secL04Tpc3/Free_ospb_colored/2'/> | ||
<scene name='G14secL04Tpc3/Free_ospb_colored/2'>Free OspB</scene> structure consists of twelve anti-parallel beta-strands followed by a single alpha helix. <scene name='G14secL04Tpc3/5-7_beta_strands_showing5/1'>Beta strands 5-7</scene> | <scene name='G14secL04Tpc3/Free_ospb_colored/2'>Free OspB</scene> structure consists of twelve anti-parallel beta-strands followed by a single alpha helix. <scene name='G14secL04Tpc3/5-7_beta_strands_showing5/1'>Beta strands 5-7</scene> | ||
and <scene name='G14secL04Tpc3/8-12_beta_strands_showing5/1'>8-12 </scene>form two sheets that bend, along with the final <scene name='G14secL04Tpc3/Alpha_helix2/1'>alpha helix </scene> | and <scene name='G14secL04Tpc3/8-12_beta_strands_showing5/1'>8-12 </scene>form two sheets that bend, along with the final <scene name='G14secL04Tpc3/Alpha_helix2/1'>alpha helix </scene> | ||
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==OspB complexed with H6831 Antibody Fab Fragment== | ==OspB complexed with H6831 Antibody Fab Fragment== | ||
<Structure load='1rjl' size='350' frame='true' align='right' caption= 'OspB bound to H6831' scene= 'G14secL04Tpc3/Ospb_in_complex/2'/> | <Structure load='1rjl' size='350' frame='true' align='right' caption= 'OspB bound to H6831 (PDB code [[1rjl]])' scene= 'G14secL04Tpc3/Ospb_in_complex/2'/> | ||
<scene name='G14secL04Tpc3/Antibody_showing2/1'>Antibody Fab fragment H6831 </scene> (<scene name='G14secL04Tpc3/Heavy_chain/1'>Heavy chain</scene> and <scene name='G14secL04Tpc3/Light_chain/1'>light chain</scene>) has been shown to bind on an epitope of the <scene name='G14secL04Tpc3/Ospb_in_complex_with_heavy_and/2'>OspB protein</scene><ref name ="1rjl_pdb" />. Once this occurs, the resulting OspB∙H6831 complex is formed and the first four beta strands of the OspB protein are proteolysed<ref name ="1rjl_pdb" />. Cleavage of these first four beta strands somehow causes bacterial lysis of the ''B. burdgorferi''. This loss is the most significant conformational change in the OspB protein itself, and all other changes are minor and appear to be related to this loss. The majority of the binding occurs on the C-terminus of OspB. The C-terminus consists of <scene name='G14secL04Tpc3/Three_major_loops2/4'>three major loops </scene> | <scene name='G14secL04Tpc3/Antibody_showing2/1'>Antibody Fab fragment H6831 </scene> (<scene name='G14secL04Tpc3/Heavy_chain/1'>Heavy chain</scene> and <scene name='G14secL04Tpc3/Light_chain/1'>light chain</scene>) has been shown to bind on an epitope of the <scene name='G14secL04Tpc3/Ospb_in_complex_with_heavy_and/2'>OspB protein</scene><ref name ="1rjl_pdb" />. Once this occurs, the resulting OspB∙H6831 complex is formed and the first four beta strands of the OspB protein are proteolysed<ref name ="1rjl_pdb" />. Cleavage of these first four beta strands somehow causes bacterial lysis of the ''B. burdgorferi''. This loss is the most significant conformational change in the OspB protein itself, and all other changes are minor and appear to be related to this loss. The majority of the binding occurs on the C-terminus of OspB. The C-terminus consists of <scene name='G14secL04Tpc3/Three_major_loops2/4'>three major loops </scene> | ||
