Luciola cruciata luciferase: Difference between revisions
From Proteopedia
Jump to navigationJump to search
Michal Harel (talk | contribs) No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
<StructureSection load='2d1r' size='500' side='right' SCENE=Luciola_cruciata_luciferase/Oxyluciferin_amp/2 caption='Structure of Luciola cruciata complexed with Oxiluciferin & AMP (PDB entry [[2d1r]])'> | |||
[[Image:2d1r.png|left|200px]] | [[Image:2d1r.png|left|200px]] | ||
[[Luciferase]] is a class of enzymes producing light through the process of bioluminescence. [[Luciola cruciata luciferase]], or Japanese firefly luciferase, catalyses a reaction of adenylation and then an oxydative decarboxylation, changing luciferin to oxyluciferin and thus emitting light. | [[Luciferase]] is a class of enzymes producing light through the process of bioluminescence. [[Luciola cruciata luciferase]], or Japanese firefly luciferase, catalyses a reaction of adenylation and then an oxydative decarboxylation, changing luciferin to oxyluciferin and thus emitting light. | ||
This protein is constituted of two main domains separated by a cleft. | This protein is constituted of two main domains separated by a cleft. | ||
| Line 20: | Line 18: | ||
==Structure== | ==Structure== | ||
2D1R is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Luciola_cruciata Luciola cruciata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1R OCA]. | |||
The protein is shown here bound with two products, adenosine 5'-phosphate (AMP) and oxyluciferine. | The protein is shown here bound with two products, adenosine 5'-phosphate (AMP) and oxyluciferine. | ||
| Line 68: | Line 66: | ||
=== Luciferase Control === | === Luciferase Control === | ||
As the structure of luciferases differ between species, so does the method of control over the bioluminescent reaction. In L. polyedrum, a marine dinoflagellate responsible for some red tides, a pH-dependant mechanism at the protein level appears to be responsible for control of bioluminescence. With fireflies however, the reaction is under at least some form of nervous control, with the insect controlling flashes through the use of nitric oxide <ref name="lights">PMID:11431567 </ref>. | As the structure of luciferases differ between species, so does the method of control over the bioluminescent reaction. In L. polyedrum, a marine dinoflagellate responsible for some red tides, a pH-dependant mechanism at the protein level appears to be responsible for control of bioluminescence. With fireflies however, the reaction is under at least some form of nervous control, with the insect controlling flashes through the use of nitric oxide <ref name="lights">PMID:11431567 </ref>. | ||
</StructureSection> | |||
__NOTOC__ | |||
==3D structures of luciferase== | ==3D structures of luciferase== | ||
