2jpp: Difference between revisions

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==Overview==

Proteins of the RsmA/CsrA family are global translational regulators in, many bacterial species. We have determined the solution structure of a, complex formed between the RsmE protein, a member of this family from, Pseudomonas fluorescens, and a target RNA encompassing the, ribosome-binding site of the hcnA gene. The RsmE homodimer with its two, RNA-binding sites makes optimal contact with an, 5'-(A)/~~(U)CANGGANG(U)~~/(A)-3' sequence in the mRNA. When tightly gripped by, RsmE, the ANGGAN core folds into a loop, favoring the formation of a, 3-base-pair stem by flanking nucleotides. We validated these findings by, in vivo and in vitro mutational analyses. The structure of the complex, explains well how, by sequestering the Shine-Dalgarno sequence, the, RsmA/CsrA proteins repress translation.

Proteins of the RsmA/CsrA family are global translational regulators in many bacterial species. We have determined the solution structure of a complex formed between the RsmE protein, a member of this family from Pseudomonas fluorescens, and a target RNA encompassing the ribosome-binding site of the hcnA gene. The RsmE homodimer with its two RNA-binding sites makes optimal contact with an 5'-A/UCANGGANGU/A-3' sequence in the mRNA. When tightly gripped by RsmE, the ANGGAN core folds into a loop, favoring the formation of a 3-base-pair stem by flanking nucleotides. We validated these findings by in vivo and in vitro mutational analyses. The structure of the complex explains well how, by sequestering the Shine-Dalgarno sequence, the RsmA/CsrA proteins repress translation.

==About this Structure==

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[[Category: Pseudomonas fluorescens]]

[[Category: Single protein]]

[[Category: Allain, F.H.T.]]

[[Category: Allain, F H.T.]]

[[Category: Duss, O.]]

[[Category: Haas, D.]]

[[Category: Jelesarov, I.]]

[[Category: Lapouge, K.]]

[[Category: Oberstrass, F.C.]]

[[Category: Oberstrass, F C.]]

[[Category: Schubert, M.]]

[[Category: csra]]

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[[Category: translation/rna complex]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Proteins of the RsmA/CsrA family are global translational regulators in, many bacterial species. We have determined the solution structure of a, complex formed between the RsmE protein, a member of this family from, Pseudomonas fluorescens, and a target RNA encompassing the, ribosome-binding site of the hcnA gene. The RsmE homodimer with its two, RNA-binding sites makes optimal contact with an, 5'-(A)/(U)CANGGANG(U)/(A)-3' sequence in the mRNA. When tightly gripped by, RsmE, the ANGGAN core folds into a loop, favoring the formation of a, 3-base-pair stem by flanking nucleotides. We validated these findings by, in vivo and in vitro mutational analyses. The structure of the complex, explains well how, by sequestering the Shine-Dalgarno sequence, the, RsmA/CsrA proteins repress translation.
+Proteins of the RsmA/CsrA family are global translational regulators in many bacterial species. We have determined the solution structure of a complex formed between the RsmE protein, a member of this family from Pseudomonas fluorescens, and a target RNA encompassing the ribosome-binding site of the hcnA gene. The RsmE homodimer with its two RNA-binding sites makes optimal contact with an 5'-A/UCANGGANGU/A-3' sequence in the mRNA. When tightly gripped by RsmE, the ANGGAN core folds into a loop, favoring the formation of a 3-base-pair stem by flanking nucleotides. We validated these findings by in vivo and in vitro mutational analyses. The structure of the complex explains well how, by sequestering the Shine-Dalgarno sequence, the RsmA/CsrA proteins repress translation.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Pseudomonas fluorescens]]
 [[Category: Single protein]]
-[[Category: Allain, F.H.T.]]
+[[Category: Allain, F H.T.]]
 [[Category: Duss, O.]]
 [[Category: Haas, D.]]
 [[Category: Jelesarov, I.]]
 [[Category: Lapouge, K.]]
-[[Category: Oberstrass, F.C.]]
+[[Category: Oberstrass, F C.]]
 [[Category: Schubert, M.]]
 [[Category: csra]]
@@ Line 27: / Line 27: @@
 [[Category: translation/rna complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 13:31:57 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:04:58 2008''