2ns1: Difference between revisions
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New page: left|200px<br /><applet load="2ns1" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ns1, resolution 1.962Å" /> '''Crystal structure o... |
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[[Image:2ns1.jpg|left|200px]]<br /><applet load="2ns1" size=" | [[Image:2ns1.jpg|left|200px]]<br /><applet load="2ns1" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2ns1, resolution 1.962Å" /> | caption="2ns1, resolution 1.962Å" /> | ||
'''Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK'''<br /> | '''Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK'''<br /> | ||
==Overview== | ==Overview== | ||
Ammonia conductance is highly regulated. A P(II) signal transduction | Ammonia conductance is highly regulated. A P(II) signal transduction protein, GlnK, is the final regulator of transmembrane ammonia conductance by the ammonia channel AmtB in Escherichia coli. The complex formed between AmtB and inhibitory GlnK at 1.96-A resolution shows that the trimeric channel is blocked directly by GlnK and how, in response to intracellular nitrogen status, the ability of GlnK to block the channel is regulated by uridylylation/deuridylylation at Y51. ATP and Mg(2+) augment the interaction of GlnK. The hydrolyzed product, adenosine 5'-diphosphate orients the surface of GlnK for AmtB blockade. 2-Oxoglutarate diminishes AmtB/GlnK association, and sites for 2-oxoglutarate are evaluated. | ||
==About this Structure== | ==About this Structure== | ||
2NS1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with BOG, ADP and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2NS1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=BOG:'>BOG</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS1 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: CSMP, Center | [[Category: CSMP, Center for Structures of Membrane Proteins.]] | ||
[[Category: Gruswitz, F.]] | [[Category: Gruswitz, F.]] | ||
[[Category: III, J | [[Category: III, J O.Connell.]] | ||
[[Category: Stroud, R | [[Category: Stroud, R M.]] | ||
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: BOG]] | [[Category: BOG]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:10:15 2008'' | ||
