2o3m: Difference between revisions
New page: left|200px<br /><applet load="2o3m" size="350" color="white" frame="true" align="right" spinBox="true" caption="2o3m" /> '''Monomeric G-DNA tetraplex from human C-kit p... |
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==Overview== | ==Overview== | ||
The c-kit oncogene is an important target in the treatment of | The c-kit oncogene is an important target in the treatment of gastrointestinal tumors. A potential approach to inhibition of the expression of this gene involves selective stabilization of G-quadruplex structures that may be induced to form in the c-kit promoter region. Here we report on the structure of an unprecedented intramolecular G-quadruplex formed by a G-rich sequence in the c-kit promoter in K+ solution. The structure represents a new folding topology with several unique features. Most strikingly, an isolated guanine is involved in G-tetrad core formation, despite the presence of four three-guanine tracts. There are four loops: two single-residue double-chain-reversal loops, a two-residue loop, and a five-residue stem-loop, which contain base-pairing alignments. This unique structural scaffold provides a highly specific platform for the future design of ligands specifically targeted to the promoter DNA of c-kit. | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Burge, S.]] | [[Category: Burge, S.]] | ||
[[Category: Kuryavyi, V | [[Category: Kuryavyi, V V.]] | ||
[[Category: Neidle, S.]] | [[Category: Neidle, S.]] | ||
[[Category: Patel, D | [[Category: Patel, D J.]] | ||
[[Category: Phan, A | [[Category: Phan, A T.]] | ||
[[Category: 5-nucleotide snap-back loops;broken strand topolog]] | [[Category: 5-nucleotide snap-back loops;broken strand topolog]] | ||
[[Category: g-tetrad;parallel g-quadruplex; edgewise]] | [[Category: g-tetrad;parallel g-quadruplex; edgewise]] | ||
[[Category: single-residue cha reversal]] | [[Category: single-residue cha reversal]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:14:07 2008'' | ||
Revision as of 16:14, 21 February 2008
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Monomeric G-DNA tetraplex from human C-kit promoter
Overview
The c-kit oncogene is an important target in the treatment of gastrointestinal tumors. A potential approach to inhibition of the expression of this gene involves selective stabilization of G-quadruplex structures that may be induced to form in the c-kit promoter region. Here we report on the structure of an unprecedented intramolecular G-quadruplex formed by a G-rich sequence in the c-kit promoter in K+ solution. The structure represents a new folding topology with several unique features. Most strikingly, an isolated guanine is involved in G-tetrad core formation, despite the presence of four three-guanine tracts. There are four loops: two single-residue double-chain-reversal loops, a two-residue loop, and a five-residue stem-loop, which contain base-pairing alignments. This unique structural scaffold provides a highly specific platform for the future design of ligands specifically targeted to the promoter DNA of c-kit.
About this Structure
2O3M is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of an unprecedented G-quadruplex scaffold in the human c-kit promoter., Phan AT, Kuryavyi V, Burge S, Neidle S, Patel DJ, J Am Chem Soc. 2007 Apr 11;129(14):4386-92. Epub 2007 Mar 16. PMID:17362008
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