2o4v: Difference between revisions

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[[Image:2o4v.gif|left|200px]]<br /><applet load="2o4v" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2o4v.gif|left|200px]]<br /><applet load="2o4v" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2o4v, resolution 1.94Å" />

'''An arginine ladder in OprP mediates phosphate specific transfer across the outer membrane'''<br />

==Overview==

The outer membrane protein OprP mediates the transport of essential, phosphate anions into the pathogenic bacterium Pseudomonas aeruginosa., Here we report the crystallographic structure of trimeric OprP at 1.9-A, resolution, revealing an unprecedented 9-residue arginine 'ladder' that, spans from the extracellular surface down through a constriction zone, where phosphate is coordinated. Lysine residues coat the inner periplasmic, surface, creating an 'electropositive sink' that pulls the phosphates, through the eyelet and into the cell.

The outer membrane protein OprP mediates the transport of essential phosphate anions into the pathogenic bacterium Pseudomonas aeruginosa. Here we report the crystallographic structure of trimeric OprP at 1.9-A resolution, revealing an unprecedented 9-residue arginine 'ladder' that spans from the extracellular surface down through a constriction zone where phosphate is coordinated. Lysine residues coat the inner periplasmic surface, creating an 'electropositive sink' that pulls the phosphates through the eyelet and into the cell.

==About this Structure==

2O4V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CA, PO4, CL, C8E and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2O4V OCA].

2O4V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=C8E:'>C8E</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O4V OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Bains, M.]]

[[Category: Hancock, R.E.]]

[[Category: Hancock, R E.]]

[[Category: Moraes, T.F.]]

[[Category: Moraes, T F.]]

[[Category: Strynadka, N.C.]]

[[Category: Strynadka, N C.]]

[[Category: C8E]]

[[Category: CA]]

Line 28:

[[Category: transport]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Wed Nov~~ 21 13:03:~~19 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:14:29 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2o4v.gif|left|200px]]<br /><applet load="2o4v" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2o4v.gif|left|200px]]<br /><applet load="2o4v" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2o4v, resolution 1.94&Aring;" />
 '''An arginine ladder in OprP mediates phosphate specific transfer across the outer membrane'''<br />
 ==Overview==
-The outer membrane protein OprP mediates the transport of essential, phosphate anions into the pathogenic bacterium Pseudomonas aeruginosa., Here we report the crystallographic structure of trimeric OprP at 1.9-A, resolution, revealing an unprecedented 9-residue arginine 'ladder' that, spans from the extracellular surface down through a constriction zone, where phosphate is coordinated. Lysine residues coat the inner periplasmic, surface, creating an 'electropositive sink' that pulls the phosphates, through the eyelet and into the cell.
+The outer membrane protein OprP mediates the transport of essential phosphate anions into the pathogenic bacterium Pseudomonas aeruginosa. Here we report the crystallographic structure of trimeric OprP at 1.9-A resolution, revealing an unprecedented 9-residue arginine 'ladder' that spans from the extracellular surface down through a constriction zone where phosphate is coordinated. Lysine residues coat the inner periplasmic surface, creating an 'electropositive sink' that pulls the phosphates through the eyelet and into the cell.
 ==About this Structure==
-O4V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CA, PO4, CL, C8E and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2O4V OCA].
+O4V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=C8E:'>C8E</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O4V OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Bains, M.]]
-[[Category: Hancock, R.E.]]
+[[Category: Hancock, R E.]]
-[[Category: Moraes, T.F.]]
+[[Category: Moraes, T F.]]
-[[Category: Strynadka, N.C.]]
+[[Category: Strynadka, N C.]]
 [[Category: C8E]]
 [[Category: CA]]
@@ Line 28: / Line 28: @@
 [[Category: transport]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:03:19 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:14:29 2008''