2of6: Difference between revisions

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==Overview==

The ~~structures~~ of immature West Nile virus particles, propagated in the, presence of ammonium chloride to block virus maturation in the low pH, environment of the trans-Golgi network, ~~were~~ determined by ~~cryoelectron,~~ microscopy. The ~~structures~~ of these particles ~~were~~ similar to immature, West Nile virus particles found as a minor component of mature virus, samples ("naturally-occurring immature particles", NOIPs). The ~~structure,~~ of mature infectious ~~flavivirus is~~ radically different ~~to that~~ of the, immature particles. The similarity of the ammonium chloride-treated, particles and NOIPs suggests that ~~either~~ the NOIPs have not undergone any, conformational change during maturation or that the conformational change, is reversible. Comparison with immature dengue virus ~~reconstructions,~~ established the ~~location~~ of the N-linked glycosylation sites of these, viruses, verifying the interpretation of the reconstructions of the, immature flaviviruses.

The structure of immature West Nile virus particles, propagated in the presence of ammonium chloride to block virus maturation in the low-pH environment of the trans-Golgi network, was determined by cryo-electron microscopy (cryo-EM). The structure of these particles was similar to that of immature West Nile virus particles found as a minor component of mature virus samples (naturally occurring immature particles [NOIPs]). The structures of mature infectious flaviviruses are radically different from those of the immature particles. The similarity of the ammonium chloride-treated particles and NOIPs suggests either that the NOIPs have not undergone any conformational change during maturation or that the conformational change is reversible. Comparison with the cryo-EM reconstruction of immature dengue virus established the locations of the N-linked glycosylation sites of these viruses, verifying the interpretation of the reconstructions of the immature flaviviruses.

==About this Structure==

Line 10:

==Reference==

Structure of ~~Immature~~ West Nile ~~Virus~~., Zhang Y, Kaufmann B, Chipman PR, Kuhn RJ, Rossmann MG, J Virol. 2007 Mar 21;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17376919 17376919]

Structure of immature West Nile virus., Zhang Y, Kaufmann B, Chipman PR, Kuhn RJ, Rossmann MG, J Virol. 2007 Jun;81(11):6141-5. Epub 2007 Mar 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17376919 17376919]

[[Category: Single protein]]

[[Category: West nile virus]]

[[Category: Chipman, P.R.]]

[[Category: Chipman, P R.]]

[[Category: Kaufmann, B.]]

[[Category: Kuhn, R.J.]]

[[Category: Kuhn, R J.]]

[[Category: Rossmann, M.G.]]

[[Category: Rossmann, M G.]]

[[Category: Zhang, Y.]]

[[Category: em immature flavivirus west nile]]

[[Category: icosahedral virus]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:17:44 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The structures of immature West Nile virus particles, propagated in the, presence of ammonium chloride to block virus maturation in the low pH, environment of the trans-Golgi network, were determined by cryoelectron, microscopy. The structures of these particles were similar to immature, West Nile virus particles found as a minor component of mature virus, samples ("naturally-occurring immature particles", NOIPs). The structure, of mature infectious flavivirus is radically different to that of the, immature particles. The similarity of the ammonium chloride-treated, particles and NOIPs suggests that either the NOIPs have not undergone any, conformational change during maturation or that the conformational change, is reversible. Comparison with immature dengue virus reconstructions, established the location of the N-linked glycosylation sites of these, viruses, verifying the interpretation of the reconstructions of the, immature flaviviruses.
+The structure of immature West Nile virus particles, propagated in the presence of ammonium chloride to block virus maturation in the low-pH environment of the trans-Golgi network, was determined by cryo-electron microscopy (cryo-EM). The structure of these particles was similar to that of immature West Nile virus particles found as a minor component of mature virus samples (naturally occurring immature particles [NOIPs]). The structures of mature infectious flaviviruses are radically different from those of the immature particles. The similarity of the ammonium chloride-treated particles and NOIPs suggests either that the NOIPs have not undergone any conformational change during maturation or that the conformational change is reversible. Comparison with the cryo-EM reconstruction of immature dengue virus established the locations of the N-linked glycosylation sites of these viruses, verifying the interpretation of the reconstructions of the immature flaviviruses.
 ==About this Structure==
@@ Line 10: / Line 10: @@
 ==Reference==
-Structure of Immature West Nile Virus., Zhang Y, Kaufmann B, Chipman PR, Kuhn RJ, Rossmann MG, J Virol. 2007 Mar 21;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17376919 17376919]
+Structure of immature West Nile virus., Zhang Y, Kaufmann B, Chipman PR, Kuhn RJ, Rossmann MG, J Virol. 2007 Jun;81(11):6141-5. Epub 2007 Mar 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17376919 17376919]
 [[Category: Single protein]]
 [[Category: West nile virus]]
-[[Category: Chipman, P.R.]]
+[[Category: Chipman, P R.]]
 [[Category: Kaufmann, B.]]
-[[Category: Kuhn, R.J.]]
+[[Category: Kuhn, R J.]]
-[[Category: Rossmann, M.G.]]
+[[Category: Rossmann, M G.]]
 [[Category: Zhang, Y.]]
 [[Category: em immature flavivirus west nile]]
 [[Category: icosahedral virus]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:04:05 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:17:44 2008''