2p6s: Difference between revisions

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==Overview==

Neisseria meningitidis, a causative agent of bacterial meningitis, has a, relatively small repertoire of transcription factors, including NMB0573, (annotated AsnC), a member of the Lrp-AsnC family of regulators that are, widely expressed in both Bacteria and Archaea. In the present study we, show that NMB0573 binds to L-leucine and L-methionine and have solved the, structure of the protein with and without bound amino acids. This has, shown, for the first time that amino acid binding does not induce, significant conformational changes in the structure of an AsnC/Lrp, regulator ~~though~~ it does appear to ~~stabilise~~ the octameric assembly of the, protein. Transcriptional profiling of wild type and NMB0573 knock-out, strains of N. meningitidis has shown that NMB0573 is associated with an, adaptive response to nutrient poor conditions reflected in a reduction in, major surface protein expression. On the basis of its structure and the, transcriptional response, we propose that NMB0573 is a global regulator in, Neisseria controlling responses to nutrient availability through, indicators of general amino acid abundance; leucine and methionine.

Neisseria meningitidis, a causative agent of bacterial meningitis, has a relatively small repertoire of transcription factors, including NMB0573 (annotated AsnC), a member of the Lrp-AsnC family of regulators that are widely expressed in both Bacteria and Archaea. In the present study we show that NMB0573 binds to l-leucine and l-methionine and have solved the structure of the protein with and without bound amino acids. This has shown, for the first time that amino acid binding does not induce significant conformational changes in the structure of an AsnC/Lrp regulator although it does appear to stabilize the octameric assembly of the protein. Transcriptional profiling of wild-type and NMB0573 knock-out strains of N. meningitidis has shown that NMB0573 is associated with an adaptive response to nutrient poor conditions reflected in a reduction in major surface protein expression. On the basis of its structure and the transcriptional response, we propose that NMB0573 is a global regulator in Neisseria controlling responses to nutrient availability through indicators of general amino acid abundance: leucine and methionine.

==About this Structure==

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==Reference==

The structure and transcriptional analysis of a global regulator from ~~neisseria~~ meningitidis., Ren J, Sainsbury S, Combs SE, Capper RG, Jordan PW, Berrow NS, Stammers DK, Saunders NJ, Owens RJ, J Biol Chem. 2007 Mar 20;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17374605 17374605]

The structure and transcriptional analysis of a global regulator from Neisseria meningitidis., Ren J, Sainsbury S, Combs SE, Capper RG, Jordan PW, Berrow NS, Stammers DK, Saunders NJ, Owens RJ, J Biol Chem. 2007 May 11;282(19):14655-64. Epub 2007 Mar 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17374605 17374605]

[[Category: Neisseria meningitidis serogroup b]]

[[Category: Single protein]]

[[Category: OPPF, Oxford.Protein.Production.Facility.]]

[[Category: OPPF, Oxford Protein Production Facility.]]

[[Category: Owens, R.J.]]

[[Category: Owens, R J.]]

[[Category: Ren, J.]]

[[Category: Sainsbury, S.]]

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[[Category: transcriptional regulator]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Neisseria meningitidis, a causative agent of bacterial meningitis, has a, relatively small repertoire of transcription factors, including NMB0573, (annotated AsnC), a member of the Lrp-AsnC family of regulators that are, widely expressed in both Bacteria and Archaea. In the present study we, show that NMB0573 binds to L-leucine and L-methionine and have solved the, structure of the protein with and without bound amino acids. This has, shown, for the first time that amino acid binding does not induce, significant conformational changes in the structure of an AsnC/Lrp, regulator though it does appear to stabilise the octameric assembly of the, protein. Transcriptional profiling of wild type and NMB0573 knock-out, strains of N. meningitidis has shown that NMB0573 is associated with an, adaptive response to nutrient poor conditions reflected in a reduction in, major surface protein expression. On the basis of its structure and the, transcriptional response, we propose that NMB0573 is a global regulator in, Neisseria controlling responses to nutrient availability through, indicators of general amino acid abundance; leucine and methionine.
+Neisseria meningitidis, a causative agent of bacterial meningitis, has a relatively small repertoire of transcription factors, including NMB0573 (annotated AsnC), a member of the Lrp-AsnC family of regulators that are widely expressed in both Bacteria and Archaea. In the present study we show that NMB0573 binds to l-leucine and l-methionine and have solved the structure of the protein with and without bound amino acids. This has shown, for the first time that amino acid binding does not induce significant conformational changes in the structure of an AsnC/Lrp regulator although it does appear to stabilize the octameric assembly of the protein. Transcriptional profiling of wild-type and NMB0573 knock-out strains of N. meningitidis has shown that NMB0573 is associated with an adaptive response to nutrient poor conditions reflected in a reduction in major surface protein expression. On the basis of its structure and the transcriptional response, we propose that NMB0573 is a global regulator in Neisseria controlling responses to nutrient availability through indicators of general amino acid abundance: leucine and methionine.
 ==About this Structure==
@@ Line 10: / Line 10: @@
 ==Reference==
-The structure and transcriptional analysis of a global regulator from neisseria meningitidis., Ren J, Sainsbury S, Combs SE, Capper RG, Jordan PW, Berrow NS, Stammers DK, Saunders NJ, Owens RJ, J Biol Chem. 2007 Mar 20;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17374605 17374605]
+The structure and transcriptional analysis of a global regulator from Neisseria meningitidis., Ren J, Sainsbury S, Combs SE, Capper RG, Jordan PW, Berrow NS, Stammers DK, Saunders NJ, Owens RJ, J Biol Chem. 2007 May 11;282(19):14655-64. Epub 2007 Mar 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17374605 17374605]
 [[Category: Neisseria meningitidis serogroup b]]
 [[Category: Single protein]]
-[[Category: OPPF, Oxford.Protein.Production.Facility.]]
+[[Category: OPPF, Oxford Protein Production Facility.]]
-[[Category: Owens, R.J.]]
+[[Category: Owens, R J.]]
 [[Category: Ren, J.]]
 [[Category: Sainsbury, S.]]
@@ Line 28: / Line 28: @@
 [[Category: transcriptional regulator]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:05:22 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:26:28 2008''