4mdx: Difference between revisions
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{{STRUCTURE_4mdx| PDB=4mdx | SCENE= }} | |||
===Crystal structure of Bacillus subtilis MazF in complex with RNA=== | |||
The | ==Function== | ||
[[http://www.uniprot.org/uniprot/ENDOA_BACSU ENDOA_BACSU]] Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli.<ref>PMID:15882409</ref> <ref>PMID:21763692</ref> | |||
==About this Structure== | |||
[[4mdx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MDX OCA]. | |||
==Reference== | |||
<references group="xtra"/><references/> | |||
[[Category: Bacillus subtilis subsp. subtilis str. 168]] | |||
[[Category: Patel, D J.]] | |||
[[Category: Simanshu, D K.]] | |||
[[Category: Antitoxin]] | |||
[[Category: Endoa]] | |||
[[Category: Hydrolase-rna complex]] | |||
[[Category: Maze]] | |||
[[Category: Mazf]] | |||
[[Category: Mrna interferase]] | |||
[[Category: Toxin]] | |||
[[Category: Toxin-antitoxin complex]] | |||
[[Category: Toxin-mrna complex]] | |||
[[Category: Ydce]] | |||
Revision as of 06:56, 23 October 2013
Crystal structure of Bacillus subtilis MazF in complex with RNA
Function
[ENDOA_BACSU] Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli.[1] [2]
About this Structure
4mdx is a 3 chain structure with sequence from Bacillus subtilis subsp. subtilis str. 168. Full crystallographic information is available from OCA.
Reference
- ↑ Pellegrini O, Mathy N, Gogos A, Shapiro L, Condon C. The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. Mol Microbiol. 2005 Jun;56(5):1139-48. PMID:15882409 doi:MMI4606
- ↑ Park JH, Yamaguchi Y, Inouye M. Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase. FEBS Lett. 2011 Aug 4;585(15):2526-32. doi: 10.1016/j.febslet.2011.07.008. Epub, 2011 Jul 13. PMID:21763692 doi:https://dx.doi.org/10.1016/j.febslet.2011.07.008
