2pkg: Difference between revisions

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==Overview==

The small t antigen (ST) of DNA tumor virus SV40 facilitates cellular, transformation by disrupting the functions of protein phosphatase 2A, (PP2A) through a poorly defined mechanism. The crystal structure of the, core domain of SV40 ST bound to the scaffolding subunit of human PP2A, reveals that the ST core domain has a novel zinc-binding fold and, interacts with the conserved ridge of HEAT repeats 3-6, which overlaps, with the binding site for the B' (also called PR61 or B56) regulatory, subunit. ST has a lower binding affinity than B' for the PP2A core enzyme., Consequently, ST does not efficiently displace B' from PP2A holoenzymes in, vitro. Notably, ST inhibits PP2A phosphatase activity through its, N-terminal J domain. These findings suggest that ST may function mainly by, inhibiting the phosphatase activity of the PP2A core enzyme, and to a, lesser extent by modulating assembly of the PP2A holoenzymes.

The small t antigen (ST) of DNA tumor virus SV40 facilitates cellular transformation by disrupting the functions of protein phosphatase 2A (PP2A) through a poorly defined mechanism. The crystal structure of the core domain of SV40 ST bound to the scaffolding subunit of human PP2A reveals that the ST core domain has a novel zinc-binding fold and interacts with the conserved ridge of HEAT repeats 3-6, which overlaps with the binding site for the B' (also called PR61 or B56) regulatory subunit. ST has a lower binding affinity than B' for the PP2A core enzyme. Consequently, ST does not efficiently displace B' from PP2A holoenzymes in vitro. Notably, ST inhibits PP2A phosphatase activity through its N-terminal J domain. These findings suggest that ST may function mainly by inhibiting the phosphatase activity of the PP2A core enzyme, and to a lesser extent by modulating assembly of the PP2A holoenzymes.

==About this Structure==

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[[Category: Protein complex]]

[[Category: Simian virus 40]]

[[Category: Jeffrey, P.D.]]

[[Category: Jeffrey, P D.]]

[[Category: Shi, Y.]]

[[Category: ZN]]

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[[Category: sv40]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The small t antigen (ST) of DNA tumor virus SV40 facilitates cellular, transformation by disrupting the functions of protein phosphatase 2A, (PP2A) through a poorly defined mechanism. The crystal structure of the, core domain of SV40 ST bound to the scaffolding subunit of human PP2A, reveals that the ST core domain has a novel zinc-binding fold and, interacts with the conserved ridge of HEAT repeats 3-6, which overlaps, with the binding site for the B' (also called PR61 or B56) regulatory, subunit. ST has a lower binding affinity than B' for the PP2A core enzyme., Consequently, ST does not efficiently displace B' from PP2A holoenzymes in, vitro. Notably, ST inhibits PP2A phosphatase activity through its, N-terminal J domain. These findings suggest that ST may function mainly by, inhibiting the phosphatase activity of the PP2A core enzyme, and to a, lesser extent by modulating assembly of the PP2A holoenzymes.
+The small t antigen (ST) of DNA tumor virus SV40 facilitates cellular transformation by disrupting the functions of protein phosphatase 2A (PP2A) through a poorly defined mechanism. The crystal structure of the core domain of SV40 ST bound to the scaffolding subunit of human PP2A reveals that the ST core domain has a novel zinc-binding fold and interacts with the conserved ridge of HEAT repeats 3-6, which overlaps with the binding site for the B' (also called PR61 or B56) regulatory subunit. ST has a lower binding affinity than B' for the PP2A core enzyme. Consequently, ST does not efficiently displace B' from PP2A holoenzymes in vitro. Notably, ST inhibits PP2A phosphatase activity through its N-terminal J domain. These findings suggest that ST may function mainly by inhibiting the phosphatase activity of the PP2A core enzyme, and to a lesser extent by modulating assembly of the PP2A holoenzymes.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Protein complex]]
 [[Category: Simian virus 40]]
-[[Category: Jeffrey, P.D.]]
+[[Category: Jeffrey, P D.]]
 [[Category: Shi, Y.]]
 [[Category: ZN]]
@@ Line 22: / Line 22: @@
 [[Category: sv40]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:47:56 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:30:22 2008''