Single stranded binding protein: Difference between revisions

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Phe-60 is an important DNA binding site. It has been shown to be the site for cross-linking.
Phe-60 is an important DNA binding site. It has been shown to be the site for cross-linking.
Tryptophan and Lysine residues are important in binding as well. “Treatments resulting in  
Tryptophan and Lysine residues are important in binding as well. Treatments resulting in  
modification of arginine, cysteine, or tyrosine residues had no effect on binding of SSB to
modification of arginine, cysteine, or tyrosine residues had no effect on binding of SSB to
DNA,  whereas modification of either lysine residues (with acetic anhydride) or tryptophan  
DNA,  whereas modification of either lysine residues (with acetic anhydride) or tryptophan  
residues (with N-bromosuccinimide) led to complete loss of binding activity” ( Meyer, 348).  
residues (with N-bromosuccinimide) led to complete loss of binding activity ( Meyer, 348).  
The two tryptophan residues involved in DNA binding are Try-40 and Try-54, which was  
The two tryptophan residues involved in DNA binding are Trp40 and Trp54, which was  
determined by mutagenesis. One more binding site was determined by site-specific mutagenesis.
determined by mutagenesis. One more binding site was determined by site-specific mutagenesis.
When His-55 is substituted with Leu it decreases binding affinity. All of these residues  
When His55 is substituted with Leu it decreases binding affinity. All of these residues  
are found in a hydrophobic region, which is suitable for nucleotide base interactions.
are found in a hydrophobic region, which is suitable for nucleotide base interactions.


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===SSB-Protein Interactions===
===SSB-Protein Interactions===


It is believed that Gly-15 may play an important role in binding the RecA protein. Mutations in Gly-15 have  
It is believed that Gly15 may play an important role in binding the RecA protein. Mutations in Gly15 have  
extreme effects on recombinational repair.  SSB has also been thought to bind with exonuclease I, DNA polymerase II,  
extreme effects on recombinational repair.  SSB has also been thought to bind with exonuclease I, DNA polymerase II,  
and a protein n, which is used to help synthesize RNA primers for the lagging strand.  
and a protein n, which is used to help synthesize RNA primers for the lagging strand.  
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