2pnf: Difference between revisions

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==Overview==

The gene product of fabG from Aquifex aeolicus has been heterologously, expressed in Escherichia coli. Purification of the protein took place, using anion-exchange and size-exclusion chromatography and the protein was, then crystallized. Diffraction data were collected to a maximum resolution, of 1.8 A and the initial phases were determined by molecular replacement., The A. aeolicus FabG protein is a putative beta-ketoacyl-acyl carrier, protein reductase. Structure-function studies of this protein are being, performed as part of a larger project investigating naturally occurring, deviations from highly conserved residues within the short-chain, oxidoreductase (SCOR) family.

The gene product of fabG from Aquifex aeolicus has been heterologously expressed in Escherichia coli. Purification of the protein took place using anion-exchange and size-exclusion chromatography and the protein was then crystallized. Diffraction data were collected to a maximum resolution of 1.8 A and the initial phases were determined by molecular replacement. The A. aeolicus FabG protein is a putative beta-ketoacyl-acyl carrier protein reductase. Structure-function studies of this protein are being performed as part of a larger project investigating naturally occurring deviations from highly conserved residues within the short-chain oxidoreductase (SCOR) family.

==About this Structure==

Line 15:

[[Category: Single protein]]

[[Category: Mao, Q.]]

[[Category: Umland, T.C.]]

[[Category: Umland, T C.]]

[[Category: 1PE]]

[[Category: MES]]

Line 21:

[[Category: short chain oxidoreductase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jan 23 15~~:29:33 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:31:17 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The gene product of fabG from Aquifex aeolicus has been heterologously, expressed in Escherichia coli. Purification of the protein took place, using anion-exchange and size-exclusion chromatography and the protein was, then crystallized. Diffraction data were collected to a maximum resolution, of 1.8 A and the initial phases were determined by molecular replacement., The A. aeolicus FabG protein is a putative beta-ketoacyl-acyl carrier, protein reductase. Structure-function studies of this protein are being, performed as part of a larger project investigating naturally occurring, deviations from highly conserved residues within the short-chain, oxidoreductase (SCOR) family.
+The gene product of fabG from Aquifex aeolicus has been heterologously expressed in Escherichia coli. Purification of the protein took place using anion-exchange and size-exclusion chromatography and the protein was then crystallized. Diffraction data were collected to a maximum resolution of 1.8 A and the initial phases were determined by molecular replacement. The A. aeolicus FabG protein is a putative beta-ketoacyl-acyl carrier protein reductase. Structure-function studies of this protein are being performed as part of a larger project investigating naturally occurring deviations from highly conserved residues within the short-chain oxidoreductase (SCOR) family.
 ==About this Structure==
@@ Line 15: / Line 15: @@
 [[Category: Single protein]]
 [[Category: Mao, Q.]]
-[[Category: Umland, T.C.]]
+[[Category: Umland, T C.]]
 [[Category: 1PE]]
 [[Category: MES]]
@@ Line 21: / Line 21: @@
 [[Category: short chain oxidoreductase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:29:33 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:31:17 2008''