2psr: Difference between revisions

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==Overview==

The crystal structure of human psoriasin (S100A7) in the native, calcium-bound form has been determined from two crystal forms of the, protein crystallized with and without divalent zinc. The overall, structures of the dimeric protein closely resemble the previously, determined holmium-substituted structure. The structures also reveal a, zinc-binding site of the protein, which is formed by three histidines and, an aspartate residue. Together, these residues coordinate the zinc ion in, a way similar to the pattern seen in certain metalloproteases and in, particular the collagenase family of proteins. Sequence comparison, suggests that this zinc site is present in a number of the remaining, members of the S100 family. The structure of S100A7 crystallized in the, absence of zinc further shows that loss of zinc results in a, reorganization of the adjacent empty and distorted EF-hand loop, causing, it to resemble a calcium-loaded EF-hand.

The crystal structure of human psoriasin (S100A7) in the native, calcium-bound form has been determined from two crystal forms of the protein crystallized with and without divalent zinc. The overall structures of the dimeric protein closely resemble the previously determined holmium-substituted structure. The structures also reveal a zinc-binding site of the protein, which is formed by three histidines and an aspartate residue. Together, these residues coordinate the zinc ion in a way similar to the pattern seen in certain metalloproteases and in particular the collagenase family of proteins. Sequence comparison suggests that this zinc site is present in a number of the remaining members of the S100 family. The structure of S100A7 crystallized in the absence of zinc further shows that loss of zinc results in a reorganization of the adjacent empty and distorted EF-hand loop, causing it to resemble a calcium-loaded EF-hand.

==About this Structure==

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[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Brodersen, D.E.]]

[[Category: Brodersen, D E.]]

[[Category: Kjeldgaard, M.]]

[[Category: Nyborg, J.]]

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[[Category: zn-binding]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:32:36 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The crystal structure of human psoriasin (S100A7) in the native, calcium-bound form has been determined from two crystal forms of the, protein crystallized with and without divalent zinc. The overall, structures of the dimeric protein closely resemble the previously, determined holmium-substituted structure. The structures also reveal a, zinc-binding site of the protein, which is formed by three histidines and, an aspartate residue. Together, these residues coordinate the zinc ion in, a way similar to the pattern seen in certain metalloproteases and in, particular the collagenase family of proteins. Sequence comparison, suggests that this zinc site is present in a number of the remaining, members of the S100 family. The structure of S100A7 crystallized in the, absence of zinc further shows that loss of zinc results in a, reorganization of the adjacent empty and distorted EF-hand loop, causing, it to resemble a calcium-loaded EF-hand.
+The crystal structure of human psoriasin (S100A7) in the native, calcium-bound form has been determined from two crystal forms of the protein crystallized with and without divalent zinc. The overall structures of the dimeric protein closely resemble the previously determined holmium-substituted structure. The structures also reveal a zinc-binding site of the protein, which is formed by three histidines and an aspartate residue. Together, these residues coordinate the zinc ion in a way similar to the pattern seen in certain metalloproteases and in particular the collagenase family of proteins. Sequence comparison suggests that this zinc site is present in a number of the remaining members of the S100 family. The structure of S100A7 crystallized in the absence of zinc further shows that loss of zinc results in a reorganization of the adjacent empty and distorted EF-hand loop, causing it to resemble a calcium-loaded EF-hand.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Brodersen, D.E.]]
+[[Category: Brodersen, D E.]]
 [[Category: Kjeldgaard, M.]]
 [[Category: Nyborg, J.]]
@@ Line 24: / Line 24: @@
 [[Category: zn-binding]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 10:46:41 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:32:36 2008''