2pyp: Difference between revisions

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[[Image:2pyp.jpg|left|200px]]<br /><applet load="2pyp" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:2pyp.jpg|left|200px]]<br /><applet load="2pyp" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2pyp, resolution 1.9Å" />

'''PHOTOACTIVE YELLOW PROTEIN, PHOTOSTATIONARY STATE, 50% GROUND STATE, 50% BLEACHED'''<br />

==Overview==

The blue-light photoreceptor photoactive yellow protein (PYP) undergoes a, self-contained light cycle. The atomic structure of the bleached signaling, intermediate in the light cycle of PYP was determined by millisecond, time-resolved, multiwavelength Laue crystallography and simultaneous, optical spectroscopy. Light-induced trans-to-cis isomerization of the, 4-hydroxycinnamyl chromophore and coupled protein rearrangements produce a, new set of active-site hydrogen bonds. An arginine gateway opens, allowing, solvent exposure and protonation of the chromophore's phenolic oxygen., Resulting changes in shape, hydrogen bonding, and electrostatic potential, at the protein surface form a likely basis for signal transduction. The, structural results suggest a general framework for the interpretation of, protein photocycles.

The blue-light photoreceptor photoactive yellow protein (PYP) undergoes a self-contained light cycle. The atomic structure of the bleached signaling intermediate in the light cycle of PYP was determined by millisecond time-resolved, multiwavelength Laue crystallography and simultaneous optical spectroscopy. Light-induced trans-to-cis isomerization of the 4-hydroxycinnamyl chromophore and coupled protein rearrangements produce a new set of active-site hydrogen bonds. An arginine gateway opens, allowing solvent exposure and protonation of the chromophore's phenolic oxygen. Resulting changes in shape, hydrogen bonding, and electrostatic potential at the protein surface form a likely basis for signal transduction. The structural results suggest a general framework for the interpretation of protein photocycles.

==About this Structure==

2PYP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila] with HC4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=2PYP OCA].

2PYP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila] with <scene name='pdbligand=HC4:'>HC4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PYP OCA].

==Reference==

Line 13:

[[Category: Halorhodospira halophila]]

[[Category: Single protein]]

[[Category: Borgstahl, G.E.O.]]

[[Category: Borgstahl, G E.O.]]

[[Category: Burke, P.]]

[[Category: Genick, U.K.]]

[[Category: Genick, U K.]]

[[Category: Getzoff, E.D.]]

[[Category: Getzoff, E D.]]

[[Category: Mcree, D.E.]]

[[Category: Mcree, D E.]]

[[Category: Moffat, K.]]

[[Category: Ng, K.]]

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[[Category: photoreceptor]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:34:17 2008''

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@@ Line 1: / Line 1: @@
-[[Image:2pyp.jpg|left|200px]]<br /><applet load="2pyp" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2pyp.jpg|left|200px]]<br /><applet load="2pyp" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2pyp, resolution 1.9&Aring;" />
 '''PHOTOACTIVE YELLOW PROTEIN, PHOTOSTATIONARY STATE, 50% GROUND STATE, 50% BLEACHED'''<br />
 ==Overview==
-The blue-light photoreceptor photoactive yellow protein (PYP) undergoes a, self-contained light cycle. The atomic structure of the bleached signaling, intermediate in the light cycle of PYP was determined by millisecond, time-resolved, multiwavelength Laue crystallography and simultaneous, optical spectroscopy. Light-induced trans-to-cis isomerization of the, 4-hydroxycinnamyl chromophore and coupled protein rearrangements produce a, new set of active-site hydrogen bonds. An arginine gateway opens, allowing, solvent exposure and protonation of the chromophore's phenolic oxygen., Resulting changes in shape, hydrogen bonding, and electrostatic potential, at the protein surface form a likely basis for signal transduction. The, structural results suggest a general framework for the interpretation of, protein photocycles.
+The blue-light photoreceptor photoactive yellow protein (PYP) undergoes a self-contained light cycle. The atomic structure of the bleached signaling intermediate in the light cycle of PYP was determined by millisecond time-resolved, multiwavelength Laue crystallography and simultaneous optical spectroscopy. Light-induced trans-to-cis isomerization of the 4-hydroxycinnamyl chromophore and coupled protein rearrangements produce a new set of active-site hydrogen bonds. An arginine gateway opens, allowing solvent exposure and protonation of the chromophore's phenolic oxygen. Resulting changes in shape, hydrogen bonding, and electrostatic potential at the protein surface form a likely basis for signal transduction. The structural results suggest a general framework for the interpretation of protein photocycles.
 ==About this Structure==
-PYP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila] with HC4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2PYP OCA].
+PYP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila] with <scene name='pdbligand=HC4:'>HC4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PYP OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Halorhodospira halophila]]
 [[Category: Single protein]]
-[[Category: Borgstahl, G.E.O.]]
+[[Category: Borgstahl, G E.O.]]
 [[Category: Burke, P.]]
-[[Category: Genick, U.K.]]
+[[Category: Genick, U K.]]
-[[Category: Getzoff, E.D.]]
+[[Category: Getzoff, E D.]]
-[[Category: Mcree, D.E.]]
+[[Category: Mcree, D E.]]
 [[Category: Moffat, K.]]
 [[Category: Ng, K.]]
@@ Line 30: / Line 30: @@
 [[Category: photoreceptor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:44:17 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:34:17 2008''