2qij: Difference between revisions

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==Overview==

Hepatitis B core (HBc) particles have been extensively exploited as, carriers for foreign immunological epitopes in the development of, multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc, particle were grown in PEG 20,000, ammonium sulfate and various types of, alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance, crystal growth. A crystal grown using MPD as a cryoprotectant diffracted, X-rays to 7.7 A resolution and data were collected to 99.6% completeness, at 8.9 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell, parameters a = 352.3, b = 465.5, c = 645.0 A. The electron-density map, reveals a protrusion that is consistent with the N-terminus extending out, from the surface of the capsid. The structure presented here supports the, idea that N-terminal insertions can be exploited in the development of, diagnostic reagents, multicomponent vaccines and delivery vehicles into, mammalian cells.

Hepatitis B core (HBc) particles have been extensively exploited as carriers for foreign immunological epitopes in the development of multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc particle were grown in PEG 20,000, ammonium sulfate and various types of alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance crystal growth. A crystal grown using MPD as a cryoprotectant diffracted X-rays to 7.7 A resolution and data were collected to 99.6% completeness at 8.9 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 352.3, b = 465.5, c = 645.0 A. The electron-density map reveals a protrusion that is consistent with the N-terminus extending out from the surface of the capsid. The structure presented here supports the idea that N-terminal insertions can be exploited in the development of diagnostic reagents, multicomponent vaccines and delivery vehicles into mammalian cells.

==About this Structure==

Line 13:

[[Category: Hepatitis b virus subtype adw]]

[[Category: Single protein]]

[[Category: Ho, K.L.]]

[[Category: Ho, K L.]]

[[Category: McNae, I.W.]]

[[Category: McNae, I W.]]

[[Category: Tan, W.S]]

[[Category: Tan, W S]]

[[Category: Walkinshaw, M.D.]]

[[Category: Walkinshaw, M D.]]

[[Category: icosahedral virus]]

[[Category: viral capsid protein]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Hepatitis B core (HBc) particles have been extensively exploited as, carriers for foreign immunological epitopes in the development of, multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc, particle were grown in PEG 20,000, ammonium sulfate and various types of, alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance, crystal growth. A crystal grown using MPD as a cryoprotectant diffracted, X-rays to 7.7 A resolution and data were collected to 99.6% completeness, at 8.9 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell, parameters a = 352.3, b = 465.5, c = 645.0 A. The electron-density map, reveals a protrusion that is consistent with the N-terminus extending out, from the surface of the capsid. The structure presented here supports the, idea that N-terminal insertions can be exploited in the development of, diagnostic reagents, multicomponent vaccines and delivery vehicles into, mammalian cells.
+Hepatitis B core (HBc) particles have been extensively exploited as carriers for foreign immunological epitopes in the development of multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc particle were grown in PEG 20,000, ammonium sulfate and various types of alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance crystal growth. A crystal grown using MPD as a cryoprotectant diffracted X-rays to 7.7 A resolution and data were collected to 99.6% completeness at 8.9 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 352.3, b = 465.5, c = 645.0 A. The electron-density map reveals a protrusion that is consistent with the N-terminus extending out from the surface of the capsid. The structure presented here supports the idea that N-terminal insertions can be exploited in the development of diagnostic reagents, multicomponent vaccines and delivery vehicles into mammalian cells.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Hepatitis b virus subtype adw]]
 [[Category: Single protein]]
-[[Category: Ho, K.L.]]
+[[Category: Ho, K L.]]
-[[Category: McNae, I.W.]]
+[[Category: McNae, I W.]]
-[[Category: Tan, W.S]]
+[[Category: Tan, W S]]
-[[Category: Walkinshaw, M.D.]]
+[[Category: Walkinshaw, M D.]]
 [[Category: icosahedral virus]]
 [[Category: viral capsid protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:44:44 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:39:42 2008''