Sandbox Reserved 779: Difference between revisions
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==Mechanism of action== | ==Mechanism of action== | ||
===Molecular mechanism of the Tanford transition=== | ===Molecular mechanism of the Tanford transition=== | ||
Above pH 6.5, b-lactoglobulin undergoes the so-called Tanford transition which is triggered by protonation of Glu89 exhibiting an anomalously | Above pH 6.5, b-lactoglobulin undergoes the so-called Tanford transition which is triggered by protonation of Glu89 exhibiting an anomalously | ||
high pKa value. The Tanford transition involves displacement of the loop EF (residues 85 to 90) that acts as a lid which closes the protein interior/binding site below pH 7.3 and opens it at higher pH. The Tanford transition may involve some other structural changes as well. For example, the transition is accompanied by a change in the microenvironment of Tyr428 and causes an alteration in the relative orientation of monomers in the dimer by as much as 5 | high pKa value. The Tanford transition involves displacement of the loop EF (residues 85 to 90) that acts as a lid which closes the protein interior/binding site below pH 7.3 and opens it at higher pH. The Tanford transition may involve some other structural changes as well. For example, the transition is accompanied by a change in the microenvironment of Tyr428 and causes an alteration in the relative orientation of monomers in the dimer by as much as 5 degrees. It should be noted that all transitions that take place between pH 2 and pH 9 do not cause any appreciable changes in the nativelike b-barrel conformation of b-lactoglobulin. | ||
<ref>PMID:11734004</ref> | <ref>PMID:11734004</ref> | ||
==Implications or possible application== | |||
describe any uses or application that have been made of the protein | |||
===how the protein function=== | |||
include chemical structure of any relevant ligands, inhibitors, or important states in the reaction pathway. | |||
==Other 3D Structures of beta-Lactoglobulin== | |||
[[8tim]], [[1tim]], [[1tpb]], [[1tpc]], [[1tpw]] – cTIM – chicken<br /> | [[8tim]], [[1tim]], [[1tpb]], [[1tpc]], [[1tpw]] – cTIM – chicken<br /> | ||
