Sandbox Reserved 763: Difference between revisions
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===Ligands=== | ===Ligands=== | ||
The liganded state of SK includes binary complexes with SO4 or MgADP ([[1l4y]]) and ternary complexes with shikimate as the first ligand and SO4 ([[2g1k]]), ADP ([[1u8a]]), MgADP, or AMPPCP (an ATP analogue) ([[1zyu]]) as the second ligand.<ref name="miscinfo" /> | The liganded state of SK includes binary complexes with SO4 or MgADP ([[1l4y]]) and ternary complexes with shikimate as the first ligand and SO4 ([[2g1k]]), ADP ([[1u8a]]), MgADP, or AMPPCP (an ATP analogue) ([[1zyu]]) as the second ligand.<ref name="miscinfo" /> | ||
Magnesium ion hold influence over the position of shikimate hydroxy groups. This ion has a role in the transfer of the γ-phosphate of ATP to the 3-hydroxy group on shikimate. Chloride ions increase enzyme affinity for ADP and ATP and help to bind the nucleotide substrate in correct orientation.<ref name="mgcl"> Effects of the magnesium and chloride ions and shikimate on the structure of shikimate kinase from Mycobacterium tuberculosis Structural Biology and Crystallization Communications | |||
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2330112/</ref> | |||
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The following methods have been used in solving for the structure of SK: | The following methods have been used in solving for the structure of SK: | ||
Crystallization | Crystallization via hanging-drop vapor-diffusion | ||
Molecular replacement methods for structure refinement | |||
==Mechanism of Action== | ==Mechanism of Action== | ||
