Sandbox Reserved 763: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 107: | Line 107: | ||
Magnesium ion hold influence over the position of shikimate hydroxy groups. This ion has a role in the transfer of the γ-phosphate of ATP to the 3-hydroxy group on shikimate. Chloride ions increase enzyme affinity for ADP and ATP and help to bind the nucleotide substrate in correct orientation.<ref name="mgcl"> Effects of the magnesium and chloride ions and shikimate on the structure of shikimate kinase from Mycobacterium tuberculosis Structural Biology and Crystallization Communications | Magnesium ion hold influence over the position of shikimate hydroxy groups. This ion has a role in the transfer of the γ-phosphate of ATP to the 3-hydroxy group on shikimate. Chloride ions increase enzyme affinity for ADP and ATP and help to bind the nucleotide substrate in correct orientation.<ref name="mgcl"> Effects of the magnesium and chloride ions and shikimate on the structure of shikimate kinase from Mycobacterium tuberculosis Structural Biology and Crystallization Communications | ||
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2330112/</ref> | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2330112/</ref> | ||
Magnesium ion binds in the active site. | |||
| Line 160: | Line 161: | ||
Crystallization via hanging-drop vapor-diffusion | Crystallization via hanging-drop vapor-diffusion | ||
Molecular replacement methods for structure refinement | Molecular replacement methods for structure refinement | ||
multiple isomorphous replacement | |||
Fourier calculations | |||
Electrospray mass spectrometry | |||
Dynamic light scattering | |||
Circular Dichroism | |||
