Sandbox Reserved 761: Difference between revisions
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*In the reductive amination reaction, ADP is a potent activator at low pH and low substrate concentration. At pH 6.0, high concentrations of α-ketoglutarate and NADH, inhibit the reaction. This substrate inhibition is alleviated by ADP. Therefore, while GTP and glutamate bind synergistically with NADH to inhibit GDH, ADP activates the reaction by decreasing the affinity of the active site. However, under conditions where the enzyme is not saturated (e.g. low substrate concentrations), this loss in binding affinity causes inhibition. <ref>D'Mello, J. P. F.. "Glutamate Dehydrogenase." Amino Acids in Human Nutrition and Health. 2012. 1-23. Print</ref>. | *In the reductive amination reaction, ADP is a potent activator at low pH and low substrate concentration. At pH 6.0, high concentrations of α-ketoglutarate and NADH, inhibit the reaction. This substrate inhibition is alleviated by ADP. Therefore, while GTP and glutamate bind synergistically with NADH to inhibit GDH, ADP activates the reaction by decreasing the affinity of the active site. However, under conditions where the enzyme is not saturated (e.g. low substrate concentrations), this loss in binding affinity causes inhibition. <ref>D'Mello, J. P. F.. "Glutamate Dehydrogenase." Amino Acids in Human Nutrition and Health. 2012. 1-23. Print</ref>. | ||
*Inhibition by high [ADP] is due to competition between ADP and the adenosine moiety of the coenzyme at the active site 1 <ref>D'Mello, J. P. F.. "Glutamate Dehydrogenase." Amino Acids in Human Nutrition and Health. 2012. 1-23. Print</ref>. | *Inhibition by high [<scene name='56/564037/Adp/1'>ADP</scene>] (Turquoise) is due to competition between ADP and the adenosine moiety of the coenzyme at the active site 1 <ref>D'Mello, J. P. F.. "Glutamate Dehydrogenase." Amino Acids in Human Nutrition and Health. 2012. 1-23. Print</ref>. | ||
'''NADH:''' | '''NADH:''' | ||
*NADH, is another major allosteric inhibitor of GDH. <ref>D'Mello, J. P. F.. "Glutamate Dehydrogenase." Amino Acids in Human Nutrition and Health. 2012. 1-23. Print</ref>. | *NADH, is another major allosteric inhibitor of GDH. <ref>D'Mello, J. P. F.. "Glutamate Dehydrogenase." Amino Acids in Human Nutrition and Health. 2012. 1-23. Print</ref>. | ||
