Sandbox Reserved 766: Difference between revisions
From Proteopedia
Jump to navigationJump to search
Louis Muccio (talk | contribs) |
Louis Muccio (talk | contribs) |
||
| Line 30: | Line 30: | ||
L-Asparaginase is a naturally occurring enzyme in bacteria that breaks down Asparagine. This easy breakdown of Asparagine into Aspartic Acid and Ammonia by L-Asparagine is able to suppress tumor cell growth cause by lymphoblastic leukemia cells by depleting the Asparagine stores creating a shortage in the plasma of the cell that cannot be compensated for potentially leading to apoptosis of the cell. (site DRL)Sometimes L-Asparaginase activity does not work to suppress the tumor cells. A recent study has been conducted to see if what could potentially cause some cells to be susceptible to treatment and others not be from within the same system. Leukemia infected cell lines were analyzed to see which ones were L-Asparaginase treatment immune and then those cell lines analyzed for protein expression and composition. It was found that in the cell lines that were immune to treatment there was an over expression of ASNS and therefore, an over expression of Asparagine to be used for maintenance and growth of the cells plasma. These findings are being evaluated and a potential new approach to treat lymphoblastic leukemia by using techniques such as RNAi silencing to suppress ASNS activity and allow L-Asparaginase to induce apoptosis in the cancerous cells. | L-Asparaginase is a naturally occurring enzyme in bacteria that breaks down Asparagine. This easy breakdown of Asparagine into Aspartic Acid and Ammonia by L-Asparagine is able to suppress tumor cell growth cause by lymphoblastic leukemia cells by depleting the Asparagine stores creating a shortage in the plasma of the cell that cannot be compensated for potentially leading to apoptosis of the cell. (site DRL)Sometimes L-Asparaginase activity does not work to suppress the tumor cells. A recent study has been conducted to see if what could potentially cause some cells to be susceptible to treatment and others not be from within the same system. Leukemia infected cell lines were analyzed to see which ones were L-Asparaginase treatment immune and then those cell lines analyzed for protein expression and composition. It was found that in the cell lines that were immune to treatment there was an over expression of ASNS and therefore, an over expression of Asparagine to be used for maintenance and growth of the cells plasma. These findings are being evaluated and a potential new approach to treat lymphoblastic leukemia by using techniques such as RNAi silencing to suppress ASNS activity and allow L-Asparaginase to induce apoptosis in the cancerous cells. | ||
====Leukemia Tumor Suppression==== | ====Leukemia Tumor Suppression==== | ||
A recent study has shown that a using a adenylated sulfoxmine transition-state analogue 1 has the ability to inhibit human ASNS. This inhibition of the continual production of Asparagine has also been able to suppress the growth of L-Asparaginase resistant Leukemia mouse cell lines. | A recent study has shown that a using a adenylated sulfoxmine transition-state analogue 1 has the ability to inhibit human ASNS. This inhibition of the continual production of Asparagine has also been able to suppress the growth of L-Asparaginase resistant Leukemia mouse cell lines.<ref>"A Sulfoximine-based Inhibitor of Human Asparagine Synthetase Kills L-asparaginase-resistant Leukemia Cells." National Center for Biotechnology Information. U.S. National Library of Medicine, n.d. Web. 04 Dec. 2013. </ref> | ||
===Ovarian Cancer Predictive Bio-Marker=== | ===Ovarian Cancer Predictive Bio-Marker=== | ||
