Sandbox Reserved 813: Difference between revisions
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==Pou-specific domain== | ==Pou-specific domain== | ||
The Pou-specific domain is located on the N-terminal site of the Oct-4 sequence and precisely from the 131th amino acid to the 205th. The POU-specific domain | The Pou-specific domain is located on the N-terminal site of the Oct-4 sequence and precisely from the 131th amino acid to the 205th. The POU-specific domain is able to bind autonomously the DNA with the binding consensus : [gAATAT(G/T)CA]. This is the thanks to a specific motif, the helix-turn-helix. At its name suggests, this pattern is composed by two α helix linked by an amino acids sequence which corresponds to the turn. The second helix is the most involved in the DNA binding. Indeed, this helix is able to carry out hydrogen bonds and Van der Waalts interactions with bases in the major groove of DNA. The first helix allows to stabilize the complex DNA-protein. | ||
==Homeodomain== | ==Homeodomain== | ||
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= References = | = References = | ||
'''POU-domain transcription factors: pou-er-ful developmental regulators.''' M G Rosenfeld ''Genes Dev. 1991 5: 897-907'' | |||
