Sandbox Reserved 829: Difference between revisions
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Pancreatic and Duodenal homeoboX 1, called PDX-1, is a transcription factor which is encoded by the gene ''Ipf1''. This one is located at the human chromosome 13q12.1. | Pancreatic and Duodenal homeoboX 1, called PDX-1, is a transcription factor which is encoded by the gene ''Ipf1''. This one is located at the human chromosome 13q12.1. | ||
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There are two complexes of the PDX-1 homeodomain with differences in the conformation of the N- terminal arm and helix 3. Indeed, the homeodomain of PDX-1 binds DNA in two different conformations named conformations A and B. | There are two complexes of the PDX-1 homeodomain with differences in the conformation of the N- terminal arm and helix 3. Indeed, the homeodomain of PDX-1 binds DNA in two different conformations named conformations A and B. | ||
Moreover, the two conformations of the Pdx1/DNA complex contained invariant contacts found in both conformations A and B, and variant contacts specific to each conformation. | Moreover, the two conformations of the Pdx1/DNA complex contained invariant contacts found in both conformations A and B, and variant contacts specific to each conformation. | ||
[[Image:Final.jpg|right|1000px|thumb|Differences between conformation A and B <ref name= "binding" />]] | |||
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[[Image:Nter.jpg|left|500px|thumb|PDX-1 homedomain/DNA interactions. | |||
A) Structure of the PDX-1 homedomain/DNA complex. | |||
B) Hydrogen bond contacts with the DNA differ between Conformation A and B. | |||
<ref name= "binding" />]] | |||
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So, the most stable configuration for the N-terminal arm of Pdx1 consists of Lys 2 inserted in the minor groove and Arg 3 outside of the minor grove contacting the phosphate backbone and Arg 43. | So, the most stable configuration for the N-terminal arm of Pdx1 consists of Lys 2 inserted in the minor groove and Arg 3 outside of the minor grove contacting the phosphate backbone and Arg 43. | ||
