Sandbox Reserved 829: Difference between revisions
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Pancreatic and Duodenal homeoboX 1, called PDX-1, is a transcription factor which is encoded by the gene ''Ipf1''. This one is located at the human chromosome 13q12.1. | Pancreatic and Duodenal homeoboX 1, called PDX-1, is a transcription factor which is encoded by the gene ''Ipf1''. This one is located at the human chromosome 13q12.1. | ||
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Human PDX-1 is a protein of 283 amino acids with a molecular weight of 30,64 kDa. This transcription factor belongs to the ParaHox transcription factor family, which diverged from the Hox subfamily through a duplication event. | Human PDX-1 is a protein of 283 amino acids with a molecular weight of 30,64 kDa. This transcription factor belongs to the ParaHox transcription factor family, which diverged from the Hox subfamily through a duplication event. | ||
Moreover, it is important to note that PDX-1 is located in the nucleus of cells. | Moreover, it is important to note that PDX-1 is located in the nucleus of cells. | ||
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<ref> PMID: 17315980 </ref> <ref name = "binding" >PMID: 23950697</ref> | <ref> PMID: 17315980 </ref> <ref name = "binding" >PMID: 23950697</ref> | ||
[[Image:Pdx1.jpg|left| | [[Image:Pdx1.jpg|left|230px|thumb|'''General structure of PDX-1 homeodomain''']] | ||
===='''Generality''' ==== | ===='''Generality''' ==== | ||
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The N-terminal arm binds with a specific DNA sequence through the minor groove. | The N-terminal arm binds with a specific DNA sequence through the minor groove. | ||
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Nuclear translocation of transcription factors is a crucial requirement for their action and stimulus-dependent nuclear translocation can serve as a mechanism to regulate gene expression at the level of transcription initiation. | Nuclear translocation of transcription factors is a crucial requirement for their action and stimulus-dependent nuclear translocation can serve as a mechanism to regulate gene expression at the level of transcription initiation. | ||
NLS is composed of several basic amino acids such as arginine (R) and lysine (K), as we can see in the NLS sequence RRMKWKK (basic amino acids) of PDX-1. This motif is sufficient for the nuclear import of PDX-1.<ref>PMID : 10567702</ref> | NLS is composed of several basic amino acids such as arginine (R) and lysine (K), as we can see in the NLS sequence RRMKWKK (basic amino acids) of PDX-1. This motif is sufficient for the nuclear import of PDX-1.<ref>PMID : 10567702</ref> | ||
