2vb3: Difference between revisions

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==Overview==

Methionine-rich motifs have an important role in copper trafficking, factors, including the CusF protein. Here we show that CusF uses a new, metal recognition site wherein Cu(I) is tetragonally displaced from a, Met2His ligand plane toward a conserved tryptophan. Spectroscopic studies, demonstrate that both thioether ligation and strong cation-pi interactions, with tryptophan stabilize metal binding. This novel active site chemistry, affords mechanisms for control of adventitious metal redox and, substitution chemistry.

Methionine-rich motifs have an important role in copper trafficking factors, including the CusF protein. Here we show that CusF uses a new metal recognition site wherein Cu(I) is tetragonally displaced from a Met2His ligand plane toward a conserved tryptophan. Spectroscopic studies demonstrate that both thioether ligation and strong cation-pi interactions with tryptophan stabilize metal binding. This novel active site chemistry affords mechanisms for control of adventitious metal redox and substitution chemistry.

==About this Structure==

2VB3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=AG:'>AG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Ag Binding Site For Chain X'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VB3 OCA].

2VB3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=AG:'>AG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Ag+Binding+Site+For+Chain+X'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VB3 OCA].

==Reference==

Line 14:

[[Category: Single protein]]

[[Category: Balakrishnan, G.]]

[[Category: Davis, A.V.]]

[[Category: Davis, A V.]]

[[Category: Focia, P.]]

[[Category: Halloran, T.V.O.]]

[[Category: Halloran, T V.O.]]

[[Category: Penner-Hahn, J.E.]]

[[Category: Penner-Hahn, J E.]]

[[Category: Spiro, T.G.]]

[[Category: Spiro, T G.]]

[[Category: Staehlin, B.M.]]

[[Category: Staehlin, B M.]]

[[Category: Stasser, J.P.]]

[[Category: Stasser, J P.]]

[[Category: Xue, Y.]]

[[Category: AG]]

Line 31:

[[Category: periplasm]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Methionine-rich motifs have an important role in copper trafficking, factors, including the CusF protein. Here we show that CusF uses a new, metal recognition site wherein Cu(I) is tetragonally displaced from a, Met2His ligand plane toward a conserved tryptophan. Spectroscopic studies, demonstrate that both thioether ligation and strong cation-pi interactions, with tryptophan stabilize metal binding. This novel active site chemistry, affords mechanisms for control of adventitious metal redox and, substitution chemistry.
+Methionine-rich motifs have an important role in copper trafficking factors, including the CusF protein. Here we show that CusF uses a new metal recognition site wherein Cu(I) is tetragonally displaced from a Met2His ligand plane toward a conserved tryptophan. Spectroscopic studies demonstrate that both thioether ligation and strong cation-pi interactions with tryptophan stabilize metal binding. This novel active site chemistry affords mechanisms for control of adventitious metal redox and substitution chemistry.
 ==About this Structure==
-VB3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=AG:'>AG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Ag Binding Site For Chain X'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VB3 OCA].
+VB3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=AG:'>AG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Ag+Binding+Site+For+Chain+X'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VB3 OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Balakrishnan, G.]]
-[[Category: Davis, A.V.]]
+[[Category: Davis, A V.]]
 [[Category: Focia, P.]]
-[[Category: Halloran, T.V.O.]]
+[[Category: Halloran, T V.O.]]
-[[Category: Penner-Hahn, J.E.]]
+[[Category: Penner-Hahn, J E.]]
-[[Category: Spiro, T.G.]]
+[[Category: Spiro, T G.]]
-[[Category: Staehlin, B.M.]]
+[[Category: Staehlin, B M.]]
-[[Category: Stasser, J.P.]]
+[[Category: Stasser, J P.]]
 [[Category: Xue, Y.]]
 [[Category: AG]]
@@ Line 31: / Line 31: @@
 [[Category: periplasm]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:13:52 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:54:44 2008''