Sandbox Reserved 828: Difference between revisions
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==E.Coli Gyrase== | |||
---- | ---- | ||
{{STRUCTURE_1ab4| PDB=1ab4 | SCENE=56/568026/Coloured/1}} | {{STRUCTURE_1ab4| PDB=1ab4 | CAPTION=A-subunit (monomer), resolution 2.80Å, 1ab4.pdb| SCENE=56/568026/Coloured/1}} | ||
[[Image:1ab4.png|left|200px]] | [[Image:1ab4.png|left|200px]] | ||
=='''Introduction :'''== | ==='''Introduction :'''=== | ||
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---- | ---- | ||
=='''Structure'''== | ==='''Structure'''=== | ||
[[Image:Gyrasegene.jpg]] | [[Image:Gyrasegene.jpg]] | ||
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---- | ---- | ||
=='''Mechanism'''== | ==='''Mechanism'''=== | ||
40-bp of duplex DNA, the G-segment, bind to the core of the enzyme and are cleaved by the active site tyrosines, while another DNA duplex, the T-segment, is captured through the ATP-induced dimerization of a protein gate, the N-gate. After passage through the transiently broken G-segment (DNA gate), the T-segment exits the protein through another protein gate, the C-gate. ATP hydrolysis and release reset the conformation of the enzyme and DNA to their initial state, poised for another strand-passage event or release of the DNA. | 40-bp of duplex DNA, the G-segment, bind to the core of the enzyme and are cleaved by the active site tyrosines, while another DNA duplex, the T-segment, is captured through the ATP-induced dimerization of a protein gate, the N-gate. After passage through the transiently broken G-segment (DNA gate), the T-segment exits the protein through another protein gate, the C-gate. ATP hydrolysis and release reset the conformation of the enzyme and DNA to their initial state, poised for another strand-passage event or release of the DNA. | ||
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=='''Reaction'''== | ==='''Reaction'''=== | ||
The process of DNA supercoiling by gyrase is the following : | The process of DNA supercoiling by gyrase is the following : | ||
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There are also others antibiotics, like the Cinodine which binds DNA and inhibits its supercoiling by gyrase. | There are also others antibiotics, like the Cinodine which binds DNA and inhibits its supercoiling by gyrase. | ||
==See Also== | ===See Also=== | ||
*[[Gyrase|Gyrase]] | *[[Gyrase|Gyrase]] | ||
*[[Topoisomerase|Topoisomerase]] | *[[Topoisomerase|Topoisomerase]] | ||
== | ===References=== | ||
<ref group="xtra">PMID:009278055</ref><references group="xtra"/> | <ref group="xtra">PMID:009278055</ref><references group="xtra"/> | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
