Sandbox Reserved 813: Difference between revisions

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==Pou-homeodomain==

The homeodomain (POUhd) is a very conserved 60 amino acid three helices alpha DNA-binding domain. We can find the homeodomain form the 223th to the 282th amino acid in the Oct-4 sequence.Its DNA sequence is called the “homeobox”, and the genes are known as “Hox gene”. The DNA recognition helix (alpha 3) binds the DNA major groove while the amino-terminal tail binds the DNA minor groove. We can draw a parallel with oct1 and oct2 about the Homeodomain and admit that the ninth residue of the DNA-recognition helix is a cysteine that might have been conserved at this position to confer DNA binding specificity , and promote more relaxed DNA sequence recognition by POU domains. The recognition helix and the inter-helix loops are rich in arginine and lysine residues, which form hydrogen bonds to the DNA backbone.

The homeodomain (POUhd) is a very conserved 60 amino acid '''three helices alpha DNA-binding domain'''. We can find the homeodomain form the 223th to the 282th amino acid in the Oct-4 sequence.Its DNA sequence is called the “homeobox”, and the genes are known as “Hox gene”. The DNA recognition helix (alpha 3) binds the DNA major groove while the amino-terminal tail binds the DNA minor groove. We can draw a parallel with oct1 and oct2 about the Homeodomain and admit that the ninth residue of the DNA-recognition helix is a cysteine that might have been conserved at this position to confer DNA binding specificity , and promote more relaxed DNA sequence recognition by POU domains. The recognition helix and the inter-helix loops are rich in arginine and lysine residues, which form hydrogen bonds to the DNA backbone.

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==In vivo==

Oct-4 is able to create a complex with Sox-2 to express genes involved in the embryonic development such as YES1. Oct-4 is also involved in the creation of intestinal and skin cancer. Indeed, the overexpression of Oct-4 provokes the upregulation of B-catenin transcription which inhibit the cellular differentiation.

Oct-4 is able to create a complex with Sox-2 to express genes involved in the embryonic development such as YES1. Oct-4 is also involved in the creation of intestinal and skin cancer. Indeed, the overexpression of Oct-4 provokes the upregulation of B-catenin [http://en.wikipedia.org/wiki/Beta-catenin] transcription which inhibit the cellular differentiation.

==In vitro==

Sandbox Reserved 813: Difference between revisions

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@@ Line 61: / Line 61: @@
 ==Pou-homeodomain==
-The homeodomain (POUhd) is a very conserved 60 amino acid three helices alpha DNA-binding domain. We can find the homeodomain form the 223th to the 282th amino acid in the Oct-4 sequence.Its DNA sequence is called the “homeobox”, and the genes are known as “Hox gene”. The DNA recognition helix (alpha 3) binds the DNA major groove while the amino-terminal tail binds the DNA minor groove. We can draw a parallel with oct1 and oct2  about the Homeodomain and admit that the ninth residue of the DNA-recognition helix is a cysteine that might have been conserved at this position to confer DNA binding specificity , and promote more relaxed DNA sequence recognition by POU domains. The recognition helix and the inter-helix loops are rich in arginine and lysine residues, which form hydrogen bonds to the DNA backbone.
+The homeodomain (POUhd) is a very conserved 60 amino acid '''three helices alpha DNA-binding domain'''. We can find the homeodomain form the 223th to the 282th amino acid in the Oct-4 sequence.Its DNA sequence is called the “homeobox”, and the genes are known as “Hox gene”. The DNA recognition helix (alpha 3) binds the DNA major groove while the amino-terminal tail binds the DNA minor groove. We can draw a parallel with oct1 and oct2  about the Homeodomain and admit that the ninth residue of the DNA-recognition helix is a cysteine that might have been conserved at this position to confer DNA binding specificity , and promote more relaxed DNA sequence recognition by POU domains. The recognition helix and the inter-helix loops are rich in arginine and lysine residues, which form hydrogen bonds to the DNA backbone.
@@ Line 67: / Line 67: @@
 ==In vivo==
-Oct-4 is able to create a complex with Sox-2 to express genes involved in the embryonic development such as YES1. Oct-4 is also involved in the creation of intestinal and skin cancer. Indeed, the overexpression of Oct-4 provokes the upregulation of B-catenin transcription which inhibit the cellular differentiation.
+Oct-4 is able to create a complex with Sox-2 to express genes involved in the embryonic development such as YES1. Oct-4 is also involved in the creation of intestinal and skin cancer. Indeed, the overexpression of Oct-4 provokes the upregulation of B-catenin [http://en.wikipedia.org/wiki/Beta-catenin] transcription which inhibit the cellular differentiation.
 ==In vitro==