2z8z: Difference between revisions

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==Overview==

The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in, a closed conformation was determined at 1.5A resolution. This structure, highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a ~~Ca(2~~+) ion, (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes, its structure and position in a closed conformation. Lid2 forms a helical, hairpin in an open conformation, but does not form it and covers the, active site in a closed conformation. Based on these results, we discuss, on the lid-opening mechanism.

The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism.

==About this Structure==

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[[Category: Matsumura, H.]]

[[Category: Takano, K.]]

[[Category: You, D.J.]]

[[Category: You, D J.]]

[[Category: CA]]

[[Category: PT]]

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[[Category: beta-roll]]

[[Category: calcium-binding protein]]

[[Category: family i.3 lipase]]

[[Category: family i 3 lipase]]

[[Category: hydrolase]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:00:51 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in, a closed conformation was determined at 1.5A resolution. This structure, highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca(2+) ion, (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes, its structure and position in a closed conformation. Lid2 forms a helical, hairpin in an open conformation, but does not form it and covers the, active site in a closed conformation. Based on these results, we discuss, on the lid-opening mechanism.
+The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism.
 ==About this Structure==
@@ Line 19: / Line 19: @@
 [[Category: Matsumura, H.]]
 [[Category: Takano, K.]]
-[[Category: You, D.J.]]
+[[Category: You, D J.]]
 [[Category: CA]]
 [[Category: PT]]
@@ Line 25: / Line 25: @@
 [[Category: beta-roll]]
 [[Category: calcium-binding protein]]
-[[Category: family i.3 lipase]]
+[[Category: family i 3 lipase]]
 [[Category: hydrolase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:40:02 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:00:51 2008''