2z9q: Difference between revisions

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==Overview==

During the ribosomal translocation, the binding of elongation factor G, (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation, of the 30S subunit relative to the 50S subunit in the direction of the, mRNA movement. By means of cryo-electron microscopy we observe that this, rotation is accompanied by a 20 A movement of the L1 stalk of the 50S, subunit, implying that this region is involved in the translocation of, deacylated tRNAs from the P to the E site. These ribosomal motions can, occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer, to the A-site tRNA or peptide removal, the presence of the charged P-site, tRNA locks the ribosome and prohibits both of these motions.

During the ribosomal translocation, the binding of elongation factor G (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S subunit in the direction of the mRNA movement. By means of cryo-electron microscopy we observe that this rotation is accompanied by a 20 A movement of the L1 stalk of the 50S subunit, implying that this region is involved in the translocation of deacylated tRNAs from the P to the E site. These ribosomal motions can occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer to the A-site tRNA or peptide removal, the presence of the charged P-site tRNA locks the ribosome and prohibits both of these motions.

==About this Structure==

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[[Category: twisted cca arm]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jan 23 12~~:55:18 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:00:58 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-During the ribosomal translocation, the binding of elongation factor G, (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation, of the 30S subunit relative to the 50S subunit in the direction of the, mRNA movement. By means of cryo-electron microscopy we observe that this, rotation is accompanied by a 20 A movement of the L1 stalk of the 50S, subunit, implying that this region is involved in the translocation of, deacylated tRNAs from the P to the E site. These ribosomal motions can, occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer, to the A-site tRNA or peptide removal, the presence of the charged P-site, tRNA locks the ribosome and prohibits both of these motions.
+During the ribosomal translocation, the binding of elongation factor G (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S subunit in the direction of the mRNA movement. By means of cryo-electron microscopy we observe that this rotation is accompanied by a 20 A movement of the L1 stalk of the 50S subunit, implying that this region is involved in the translocation of deacylated tRNAs from the P to the E site. These ribosomal motions can occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer to the A-site tRNA or peptide removal, the presence of the charged P-site tRNA locks the ribosome and prohibits both of these motions.
 ==About this Structure==
@@ Line 19: / Line 19: @@
 [[Category: twisted cca arm]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:55:18 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:00:58 2008''