333d: Difference between revisions
New page: left|200px<br /><applet load="333d" size="350" color="white" frame="true" align="right" spinBox="true" caption="333d, resolution 2.520Å" /> '''THE CRYSTAL STRUCTU... |
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==Overview== | ==Overview== | ||
The X-ray crystallographic structure of the RNA duplex [r(CGCAIGCG)]2 has | The X-ray crystallographic structure of the RNA duplex [r(CGCAIGCG)]2 has been refined to 2.5 A. It shows a symmetric internal loop of two non-Watson-Crick base pairs which form in the middle of the duplex. The tandem A-I/I-A pairs are related by a crystallographic two-fold axis. Both A(anti)-I(anti) mismatches are in a head-to-head conformation forming hydrogen bonds using the Watson-Crick positions. The octamer duplexes stack above one another in the cell forming a pseudo-infinite helix throughout the crystal. A hydrated calcium ion bridges between the 3'-terminal of one molecule and the backbone of another. The tandem A-I mismatches are incorporated with only minor distortion to the backbone. This is in contrast to the large helical perturbations often produced by sheared G-A pairs in RNA oligonucleotides. | ||
==About this Structure== | ==About this Structure== | ||
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The crystal structure of an RNA oligomer incorporating tandem adenosine-inosine mismatches., Carter RJ, Baeyens KJ, SantaLucia J, Turner DH, Holbrook SR, Nucleic Acids Res. 1997 Oct 15;25(20):4117-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9321667 9321667] | The crystal structure of an RNA oligomer incorporating tandem adenosine-inosine mismatches., Carter RJ, Baeyens KJ, SantaLucia J, Turner DH, Holbrook SR, Nucleic Acids Res. 1997 Oct 15;25(20):4117-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9321667 9321667] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Baeyens, K | [[Category: Baeyens, K J.]] | ||
[[Category: Carter, R | [[Category: Carter, R J.]] | ||
[[Category: Holbrook, S | [[Category: Holbrook, S R.]] | ||
[[Category: Jr., J | [[Category: Jr., J SantaLucia.]] | ||
[[Category: Turner, D | [[Category: Turner, D H.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
[[Category: a-rna]] | [[Category: a-rna]] | ||
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[[Category: mismatched]] | [[Category: mismatched]] | ||
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Revision as of 17:02, 21 February 2008
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THE CRYSTAL STRUCTURE OF AN RNA OLIGOMER INCORPORATING TANDEM ADENOSINE-INOSINE MISMATCHES
Overview
The X-ray crystallographic structure of the RNA duplex [r(CGCAIGCG)]2 has been refined to 2.5 A. It shows a symmetric internal loop of two non-Watson-Crick base pairs which form in the middle of the duplex. The tandem A-I/I-A pairs are related by a crystallographic two-fold axis. Both A(anti)-I(anti) mismatches are in a head-to-head conformation forming hydrogen bonds using the Watson-Crick positions. The octamer duplexes stack above one another in the cell forming a pseudo-infinite helix throughout the crystal. A hydrated calcium ion bridges between the 3'-terminal of one molecule and the backbone of another. The tandem A-I mismatches are incorporated with only minor distortion to the backbone. This is in contrast to the large helical perturbations often produced by sheared G-A pairs in RNA oligonucleotides.
About this Structure
333D is a Protein complex structure of sequences from [1] with CA as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of an RNA oligomer incorporating tandem adenosine-inosine mismatches., Carter RJ, Baeyens KJ, SantaLucia J, Turner DH, Holbrook SR, Nucleic Acids Res. 1997 Oct 15;25(20):4117-22. PMID:9321667
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