359d: Difference between revisions

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==Overview==

Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by, competing with a single magnesium(II) ion. X-ray crystallography revealed, that the Tb(III) ion binds to a site adjacent to an essential guanosine in, the catalytic core of the ribozyme, approximately 10 angstroms from the, cleavage site. Synthetic modifications near this binding site yielded an, RNA substrate that was resistant to Tb(III) binding and capable of being, cleaved, even in the presence of up to 20 micromolar Tb(III). It is, suggested that the magnesium(II) ion thought to bind at this site may act, as a switch, affecting the conformational changes required to achieve the, transition state.

Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by competing with a single magnesium(II) ion. X-ray crystallography revealed that the Tb(III) ion binds to a site adjacent to an essential guanosine in the catalytic core of the ribozyme, approximately 10 angstroms from the cleavage site. Synthetic modifications near this binding site yielded an RNA substrate that was resistant to Tb(III) binding and capable of being cleaved, even in the presence of up to 20 micromolar Tb(III). It is suggested that the magnesium(II) ion thought to bind at this site may act as a switch, affecting the conformational changes required to achieve the transition state.

==About this Structure==

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Inhibition of the hammerhead ribozyme cleavage reaction by site-specific binding of Tb., Feig AL, Scott WG, Uhlenbeck OC, Science. 1998 Jan 2;279(5347):81-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9417029 9417029]

[[Category: Protein complex]]

[[Category: Feig, A.L.]]

[[Category: Feig, A L.]]

[[Category: Scott, W.G.]]

[[Category: Scott, W G.]]

[[Category: Uhlenbeck, O.C.]]

[[Category: Uhlenbeck, O C.]]

[[Category: TB]]

[[Category: catalytic rna]]

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[[Category: rna hammerhead ribozyme]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by, competing with a single magnesium(II) ion. X-ray crystallography revealed, that the Tb(III) ion binds to a site adjacent to an essential guanosine in, the catalytic core of the ribozyme, approximately 10 angstroms from the, cleavage site. Synthetic modifications near this binding site yielded an, RNA substrate that was resistant to Tb(III) binding and capable of being, cleaved, even in the presence of up to 20 micromolar Tb(III). It is, suggested that the magnesium(II) ion thought to bind at this site may act, as a switch, affecting the conformational changes required to achieve the, transition state.
+Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by competing with a single magnesium(II) ion. X-ray crystallography revealed that the Tb(III) ion binds to a site adjacent to an essential guanosine in the catalytic core of the ribozyme, approximately 10 angstroms from the cleavage site. Synthetic modifications near this binding site yielded an RNA substrate that was resistant to Tb(III) binding and capable of being cleaved, even in the presence of up to 20 micromolar Tb(III). It is suggested that the magnesium(II) ion thought to bind at this site may act as a switch, affecting the conformational changes required to achieve the transition state.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 Inhibition of the hammerhead ribozyme cleavage reaction by site-specific binding of Tb., Feig AL, Scott WG, Uhlenbeck OC, Science. 1998 Jan 2;279(5347):81-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9417029 9417029]
 [[Category: Protein complex]]
-[[Category: Feig, A.L.]]
+[[Category: Feig, A L.]]
-[[Category: Scott, W.G.]]
+[[Category: Scott, W G.]]
-[[Category: Uhlenbeck, O.C.]]
+[[Category: Uhlenbeck, O C.]]
 [[Category: TB]]
 [[Category: catalytic rna]]
@@ Line 20: / Line 20: @@
 [[Category: rna hammerhead ribozyme]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:36:35 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:25 2008''