378d: Difference between revisions

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==Overview==

The 2.40 A resolution crystal structure of a side-by-side binding of, distamycin A molecules to a DNA octamer d(GTATATAC)2 with an extended, alternating TA sequence has been determined. The unit-cell parameters are, a = 29.55, b = 42.18, c = 43.38 A, beta = 96.56 degrees, space group P21, with two molecules in the asymmetric unit, in contrast to all previous, side-by-side distamycin-DNA complexes which have only a single DNA strand, and one drug molecule in the asymmetric unit. The structure was solved by, the molecular-replacement method and refined to an R index of 21.0% using, 3467 reflections [>/= 2sigma(F)]. The minor grooves of the DNA molecules, bind two side-by-side antiparallel staggered distamycins spanning about, five base pairs and virtually covering the entire length of the DNA. The, octamer duplexes exhibit low-high alternations in the helical twist, sugar, puckering and the C-O3' and O3'-P torsion angles, similar to the earlier, side-by-side complexes containing inosine bases. The molecules are stacked, one over the other along the ac diagonal in an infinite pseudo-continuous, helical column with no lateral interactions.

The 2.40 A resolution crystal structure of a side-by-side binding of distamycin A molecules to a DNA octamer d(GTATATAC)2 with an extended alternating TA sequence has been determined. The unit-cell parameters are a = 29.55, b = 42.18, c = 43.38 A, beta = 96.56 degrees, space group P21, with two molecules in the asymmetric unit, in contrast to all previous side-by-side distamycin-DNA complexes which have only a single DNA strand and one drug molecule in the asymmetric unit. The structure was solved by the molecular-replacement method and refined to an R index of 21.0% using 3467 reflections [>/= 2sigma(F)]. The minor grooves of the DNA molecules bind two side-by-side antiparallel staggered distamycins spanning about five base pairs and virtually covering the entire length of the DNA. The octamer duplexes exhibit low-high alternations in the helical twist, sugar puckering and the C-O3' and O3'-P torsion angles, similar to the earlier side-by-side complexes containing inosine bases. The molecules are stacked one over the other along the ac diagonal in an infinite pseudo-continuous helical column with no lateral interactions.

==About this Structure==

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Structure of the side-by-side binding of distamycin to d(GTATATAC)2., Mitra SN, Wahl MC, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):602-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089456 10089456]

[[Category: Protein complex]]

[[Category: Mitra, S.N.]]

[[Category: Mitra, S N.]]

[[Category: Sundaralingam, M.]]

[[Category: Wahl, M.C.]]

[[Category: Wahl, M C.]]

[[Category: DMY]]

[[Category: NA]]

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[[Category: right handed dna]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The 2.40 A resolution crystal structure of a side-by-side binding of, distamycin A molecules to a DNA octamer d(GTATATAC)2 with an extended, alternating TA sequence has been determined. The unit-cell parameters are, a = 29.55, b = 42.18, c = 43.38 A, beta = 96.56 degrees, space group P21, with two molecules in the asymmetric unit, in contrast to all previous, side-by-side distamycin-DNA complexes which have only a single DNA strand, and one drug molecule in the asymmetric unit. The structure was solved by, the molecular-replacement method and refined to an R index of 21.0% using, 3467 reflections [&gt;/= 2sigma(F)]. The minor grooves of the DNA molecules, bind two side-by-side antiparallel staggered distamycins spanning about, five base pairs and virtually covering the entire length of the DNA. The, octamer duplexes exhibit low-high alternations in the helical twist, sugar, puckering and the C-O3' and O3'-P torsion angles, similar to the earlier, side-by-side complexes containing inosine bases. The molecules are stacked, one over the other along the ac diagonal in an infinite pseudo-continuous, helical column with no lateral interactions.
+The 2.40 A resolution crystal structure of a side-by-side binding of distamycin A molecules to a DNA octamer d(GTATATAC)2 with an extended alternating TA sequence has been determined. The unit-cell parameters are a = 29.55, b = 42.18, c = 43.38 A, beta = 96.56 degrees, space group P21, with two molecules in the asymmetric unit, in contrast to all previous side-by-side distamycin-DNA complexes which have only a single DNA strand and one drug molecule in the asymmetric unit. The structure was solved by the molecular-replacement method and refined to an R index of 21.0% using 3467 reflections [&gt;/= 2sigma(F)]. The minor grooves of the DNA molecules bind two side-by-side antiparallel staggered distamycins spanning about five base pairs and virtually covering the entire length of the DNA. The octamer duplexes exhibit low-high alternations in the helical twist, sugar puckering and the C-O3' and O3'-P torsion angles, similar to the earlier side-by-side complexes containing inosine bases. The molecules are stacked one over the other along the ac diagonal in an infinite pseudo-continuous helical column with no lateral interactions.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 Structure of the side-by-side binding of distamycin to d(GTATATAC)2., Mitra SN, Wahl MC, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):602-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089456 10089456]
 [[Category: Protein complex]]
-[[Category: Mitra, S.N.]]
+[[Category: Mitra, S N.]]
 [[Category: Sundaralingam, M.]]
-[[Category: Wahl, M.C.]]
+[[Category: Wahl, M C.]]
 [[Category: DMY]]
 [[Category: NA]]
@@ Line 21: / Line 21: @@
 [[Category: right handed dna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:37:14 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:26 2008''