3cmh: Difference between revisions

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[[Image:3cmh.jpg|left|200px]]<br /><applet load="3cmh" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:3cmh.jpg|left|200px]]<br /><applet load="3cmh" size="350" color="white" frame="true" align="right" spinBox="true"

caption="3cmh" />

'''SYNTHETIC LINEAR TRUNCATED ENDOTHELIN-1 AGONIST'''<br />

==Overview==

The solution structure of a biologically active modified linear, endothelin-1 analogue, ET1-21[Cys(Acm)1,15, Aib3,11, Leu7], has been, determined for the first time by two-dimensional nuclear magnetic, resonance spectroscopy in a methanol-d3/water solvent mixture. Out of, approximately one hundred linear peptide analogues tested by biological, assay, this peptide, together with a dozen others, showed significant ETB, selective agonist activity. Here we report the solution structure of an, ETB selective agonist of a full-length, synthetic linear endothelin, analogue. The calculated structures indicate that the peptide adopts an, alpha-helical conformation between residues Ser5-His16, whilst both N- and, C-termini show no preferred conformation. These results suggest that the, disulphide bridges normally associated with endothelin and sarafotoxin, peptides may not necessarily be important for either ETB receptor binding, activity or the formation of a helical conformation in solution.

The solution structure of a biologically active modified linear endothelin-1 analogue, ET1-21[Cys(Acm)1,15, Aib3,11, Leu7], has been determined for the first time by two-dimensional nuclear magnetic resonance spectroscopy in a methanol-d3/water solvent mixture. Out of approximately one hundred linear peptide analogues tested by biological assay, this peptide, together with a dozen others, showed significant ETB selective agonist activity. Here we report the solution structure of an ETB selective agonist of a full-length, synthetic linear endothelin analogue. The calculated structures indicate that the peptide adopts an alpha-helical conformation between residues Ser5-His16, whilst both N- and C-termini show no preferred conformation. These results suggest that the disulphide bridges normally associated with endothelin and sarafotoxin peptides may not necessarily be important for either ETB receptor binding activity or the formation of a helical conformation in solution.

==About this Structure==

3CMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=3CMH OCA].

3CMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CMH OCA].

==Reference==

Solution structure of a novel ETB receptor selective agonist ET1-21 [Cys(Acm)1,15, Aib3,11, Leu7] by nuclear magnetic resonance spectroscopy and molecular modelling., Hewage CM, Jiang L, Parkinson JA, Ramage R, Sadler IH, J Pept Res. 1999 Mar;53(3):223-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10231710 10231710]

[[Category: Single protein]]

[[Category: Hewage, C.M.]]

[[Category: Hewage, C M.]]

[[Category: Jiang, L.]]

[[Category: Parkinson, J.A.]]

[[Category: Parkinson, J A.]]

[[Category: Ramage, R.]]

[[Category: Sadler, I.H.]]

[[Category: Sadler, I H.]]

[[Category: endothelin-1]]

[[Category: vasoconstrictor]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 19:35:~~28 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:08:49 2008''

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@@ Line 1: / Line 1: @@
-[[Image:3cmh.jpg|left|200px]]<br /><applet load="3cmh" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:3cmh.jpg|left|200px]]<br /><applet load="3cmh" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="3cmh" />
 '''SYNTHETIC LINEAR TRUNCATED ENDOTHELIN-1 AGONIST'''<br />
 ==Overview==
-The solution structure of a biologically active modified linear, endothelin-1 analogue, ET1-21[Cys(Acm)1,15, Aib3,11, Leu7], has been, determined for the first time by two-dimensional nuclear magnetic, resonance spectroscopy in a methanol-d3/water solvent mixture. Out of, approximately one hundred linear peptide analogues tested by biological, assay, this peptide, together with a dozen others, showed significant ETB, selective agonist activity. Here we report the solution structure of an, ETB selective agonist of a full-length, synthetic linear endothelin, analogue. The calculated structures indicate that the peptide adopts an, alpha-helical conformation between residues Ser5-His16, whilst both N- and, C-termini show no preferred conformation. These results suggest that the, disulphide bridges normally associated with endothelin and sarafotoxin, peptides may not necessarily be important for either ETB receptor binding, activity or the formation of a helical conformation in solution.
+The solution structure of a biologically active modified linear endothelin-1 analogue, ET1-21[Cys(Acm)1,15, Aib3,11, Leu7], has been determined for the first time by two-dimensional nuclear magnetic resonance spectroscopy in a methanol-d3/water solvent mixture. Out of approximately one hundred linear peptide analogues tested by biological assay, this peptide, together with a dozen others, showed significant ETB selective agonist activity. Here we report the solution structure of an ETB selective agonist of a full-length, synthetic linear endothelin analogue. The calculated structures indicate that the peptide adopts an alpha-helical conformation between residues Ser5-His16, whilst both N- and C-termini show no preferred conformation. These results suggest that the disulphide bridges normally associated with endothelin and sarafotoxin peptides may not necessarily be important for either ETB receptor binding activity or the formation of a helical conformation in solution.
 ==About this Structure==
-CMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3CMH OCA].
+CMH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CMH OCA].
 ==Reference==
 Solution structure of a novel ETB receptor selective agonist ET1-21 [Cys(Acm)1,15, Aib3,11, Leu7] by nuclear magnetic resonance spectroscopy and molecular modelling., Hewage CM, Jiang L, Parkinson JA, Ramage R, Sadler IH, J Pept Res. 1999 Mar;53(3):223-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10231710 10231710]
 [[Category: Single protein]]
-[[Category: Hewage, C.M.]]
+[[Category: Hewage, C M.]]
 [[Category: Jiang, L.]]
-[[Category: Parkinson, J.A.]]
+[[Category: Parkinson, J A.]]
 [[Category: Ramage, R.]]
-[[Category: Sadler, I.H.]]
+[[Category: Sadler, I H.]]
 [[Category: endothelin-1]]
 [[Category: vasoconstrictor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:35:28 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:08:49 2008''