3lkf: Difference between revisions

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[[Image:3lkf.jpg|left|200px]]<br /><applet load="3lkf" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:3lkf.jpg|left|200px]]<br /><applet load="3lkf" size="350" color="white" frame="true" align="right" spinBox="true"

caption="3lkf, resolution 1.90Å" />

'''LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS WITH PHOSPHOCHOLINE BOUND'''<br />

==Overview==

Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are, self-assembling, channel-forming proteins related in sequence and, function. In the alpha-hemolysin heptamer, the channel-forming, beta-strands and the amino latch make long excursions from the protomer, core. Here we report the crystal structure of the water soluble form of, LukF. In the LukF structure the channel-forming region folds into an, amphipathic, three-strand beta-sheet and the amino latch forms a, beta-strand extending a central beta-sheet. The LukF structure illustrates, how a channel-forming toxin masks protein-protein and protein-membrane, interfaces prior to cell binding and assembly, and together with the, alpha-hemolysin heptamer structure, they define the end points on the, pathway of toxin assembly.

Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are self-assembling, channel-forming proteins related in sequence and function. In the alpha-hemolysin heptamer, the channel-forming beta-strands and the amino latch make long excursions from the protomer core. Here we report the crystal structure of the water soluble form of LukF. In the LukF structure the channel-forming region folds into an amphipathic, three-strand beta-sheet and the amino latch forms a beta-strand extending a central beta-sheet. The LukF structure illustrates how a channel-forming toxin masks protein-protein and protein-membrane interfaces prior to cell binding and assembly, and together with the alpha-hemolysin heptamer structure, they define the end points on the pathway of toxin assembly.

==About this Structure==

3LKF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with PC as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=3LKF OCA].

3LKF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=PC:'>PC</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKF OCA].

==Reference==

Line 13:

[[Category: Single protein]]

[[Category: Staphylococcus aureus]]

[[Category: Gouaux, J.E.]]

[[Category: Gouaux, J E.]]

[[Category: Kamio, Y.]]

[[Category: Nariya, H.]]

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[[Category: pore-forming toxin]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 19:49:~~24 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:10:04 2008''

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@@ Line 1: / Line 1: @@
-[[Image:3lkf.jpg|left|200px]]<br /><applet load="3lkf" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:3lkf.jpg|left|200px]]<br /><applet load="3lkf" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="3lkf, resolution 1.90&Aring;" />
 '''LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS WITH PHOSPHOCHOLINE BOUND'''<br />
 ==Overview==
-Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are, self-assembling, channel-forming proteins related in sequence and, function. In the alpha-hemolysin heptamer, the channel-forming, beta-strands and the amino latch make long excursions from the protomer, core. Here we report the crystal structure of the water soluble form of, LukF. In the LukF structure the channel-forming region folds into an, amphipathic, three-strand beta-sheet and the amino latch forms a, beta-strand extending a central beta-sheet. The LukF structure illustrates, how a channel-forming toxin masks protein-protein and protein-membrane, interfaces prior to cell binding and assembly, and together with the, alpha-hemolysin heptamer structure, they define the end points on the, pathway of toxin assembly.
+Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are self-assembling, channel-forming proteins related in sequence and function. In the alpha-hemolysin heptamer, the channel-forming beta-strands and the amino latch make long excursions from the protomer core. Here we report the crystal structure of the water soluble form of LukF. In the LukF structure the channel-forming region folds into an amphipathic, three-strand beta-sheet and the amino latch forms a beta-strand extending a central beta-sheet. The LukF structure illustrates how a channel-forming toxin masks protein-protein and protein-membrane interfaces prior to cell binding and assembly, and together with the alpha-hemolysin heptamer structure, they define the end points on the pathway of toxin assembly.
 ==About this Structure==
-LKF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with PC as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3LKF OCA].
+LKF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=PC:'>PC</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKF OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Staphylococcus aureus]]
-[[Category: Gouaux, J.E.]]
+[[Category: Gouaux, J E.]]
 [[Category: Kamio, Y.]]
 [[Category: Nariya, H.]]
@@ Line 23: / Line 23: @@
 [[Category: pore-forming toxin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 19:49:24 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:10:04 2008''