401d: Difference between revisions

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==Overview==

The X-ray crystal structure of the DNA decamer d(GACCGCGGTC), containing, half the human papilloma virus E2 binding site, has been solved from two, crystals grown at different ionic conditions (50 mM MgCl2and 50 mM, spermine or 1.56 mM MgCl2and 1.56 mM spermine). Despite the variation in, salt concentration, the two DNA structures are in a very similar, A-type, DNA conformation, with helical axes curving towards the major groove., Although the salt concentrations do not effect the helical parameters or, hydration to a large degree, there is a change in the overall helical, curvature; 18 degrees and 31 degrees for the low and high salt structures, respectively. This curvature appears to be sequence specific and, biologically relevant when compared with similar DNA structures, including, the E2 binding site of a protein-DNA complex.

The X-ray crystal structure of the DNA decamer d(GACCGCGGTC), containing half the human papilloma virus E2 binding site, has been solved from two crystals grown at different ionic conditions (50 mM MgCl2and 50 mM spermine or 1.56 mM MgCl2and 1.56 mM spermine). Despite the variation in salt concentration, the two DNA structures are in a very similar, A-type DNA conformation, with helical axes curving towards the major groove. Although the salt concentrations do not effect the helical parameters or hydration to a large degree, there is a change in the overall helical curvature; 18 degrees and 31 degrees for the low and high salt structures, respectively. This curvature appears to be sequence specific and biologically relevant when compared with similar DNA structures, including the E2 binding site of a protein-DNA complex.

==About this Structure==

Line 12:

X-ray crystal structures of half the human papilloma virus E2 binding site: d(GACCGCGGTC)., Finley JB, Luo M, Nucleic Acids Res. 1998 Dec 15;26(24):5719-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9838004 9838004]

[[Category: Protein complex]]

[[Category: Finley, J.B.]]

[[Category: Finley, J B.]]

[[Category: Luo, M.]]

[[Category: dna double helix]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:11:52 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The X-ray crystal structure of the DNA decamer d(GACCGCGGTC), containing, half the human papilloma virus E2 binding site, has been solved from two, crystals grown at different ionic conditions (50 mM MgCl2and 50 mM, spermine or 1.56 mM MgCl2and 1.56 mM spermine). Despite the variation in, salt concentration, the two DNA structures are in a very similar, A-type, DNA conformation, with helical axes curving towards the major groove., Although the salt concentrations do not effect the helical parameters or, hydration to a large degree, there is a change in the overall helical, curvature; 18 degrees and 31 degrees for the low and high salt structures, respectively. This curvature appears to be sequence specific and, biologically relevant when compared with similar DNA structures, including, the E2 binding site of a protein-DNA complex.
+The X-ray crystal structure of the DNA decamer d(GACCGCGGTC), containing half the human papilloma virus E2 binding site, has been solved from two crystals grown at different ionic conditions (50 mM MgCl2and 50 mM spermine or 1.56 mM MgCl2and 1.56 mM spermine). Despite the variation in salt concentration, the two DNA structures are in a very similar, A-type DNA conformation, with helical axes curving towards the major groove. Although the salt concentrations do not effect the helical parameters or hydration to a large degree, there is a change in the overall helical curvature; 18 degrees and 31 degrees for the low and high salt structures, respectively. This curvature appears to be sequence specific and biologically relevant when compared with similar DNA structures, including the E2 binding site of a protein-DNA complex.
 ==About this Structure==
@@ Line 12: / Line 12: @@
 X-ray crystal structures of half the human papilloma virus E2 binding site: d(GACCGCGGTC)., Finley JB, Luo M, Nucleic Acids Res. 1998 Dec 15;26(24):5719-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9838004 9838004]
 [[Category: Protein complex]]
-[[Category: Finley, J.B.]]
+[[Category: Finley, J B.]]
 [[Category: Luo, M.]]
 [[Category: dna double helix]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:42:02 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:11:52 2008''