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'''Unreleased structure'''
{{STRUCTURE_4cke|  PDB=4cke  |  SCENE=  }}
===Vaccinia virus capping enzyme complexed with SAH in P1 form===
{{ABSTRACT_PUBMED_24607143}}


The entry 4cke is ON HOLD until Paper Publication
==Function==
[[http://www.uniprot.org/uniprot/MCEL_VACCW MCEL_VACCW]] Catalytic subunit of the mRNA capping enzyme which catalyzes three enzymatic reactions: the 5' triphosphate end of the pre-mRNA is hydrolyzed to a diphosphate by RNA 5' triphosphatase; the diphosphate RNA end is capped with GMP by RNA guanylyltransferase and the GpppN cap is methylated by RNA (guanine-N7) methyltransferase. Heterodimeric mRNA capping enzyme catalyzes the linkage of a N7-methyl-guanosine moiety to the first transcribed nucleotide (cap 0 structure), whereas the polymerase associated VP39 is responsible for a second methylation at the 2'-O position of the ribose (cap 1 structure).<ref>PMID:18295814</ref> <ref>PMID:18455214</ref> <ref>PMID:18256245</ref>  The heterodimeric enzyme is also involved in early viral gene transcription termination and intermediate viral gene transcription initiation. Early gene transcription termination requires the termination factor VTF, the DNA-dependent ATPase NPH-I and the Rap94 subunit of the viral RNA polymerase, as well as the presence of a specific termination motif. Binds, together with RAP94, to the termination motif 5'-UUUUUNU-3' in the nascent early mRNA.<ref>PMID:18295814</ref> <ref>PMID:18455214</ref> <ref>PMID:18256245</ref>  [[http://www.uniprot.org/uniprot/MCES_VACCW MCES_VACCW]] Regulatory subunit of the mRNA cap enzyme which stabilizes the catalytic subunit and enhances its methyltransferase activity through an allosteric mechanism. Heterodimeric mRNA capping enzyme catalyzes the linkage of a N7-methyl-guanosine moiety to the first transcribed nucleotide (cap 0 structure), whereas the polymerase associated VP39 is responsible for a second methylation at the 2'-O position of the ribose (cap 1 structure).<ref>PMID:2552660</ref> <ref>PMID:18295814</ref> <ref>PMID:18455214</ref>  The heterodimeric enzyme is also involved in early viral gene transcription termination and intermediate viral gene transcription initiation. Early gene transcription termination requires the termination factor VTF, the DNA-dependent ATPase NPH-I and the Rap94 subunit of the viral RNA polymerase, as well as the presence of a specific termination motif. Binds, together with RAP94, to the termination motif 5'-UUUUUNU-3' in the nascent early mRNA.<ref>PMID:2552660</ref> <ref>PMID:18295814</ref> <ref>PMID:18455214</ref>  


Authors: Kyrieleis, O.J.P., Chang, J., de la Pena, M., Shuman, S., Cusack, S.
==About this Structure==
[[4cke]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CKE OCA].  


Description: Vaccinia virus capping enzyme complexed with SAH in P1 form
==Reference==
<ref group="xtra">PMID:024607143</ref><references group="xtra"/><references/>
[[Category: Chang, J.]]
[[Category: Cusack, S.]]
[[Category: Kyrieleis, O J.P.]]
[[Category: Pena, M de la.]]
[[Category: Shuman, S.]]
[[Category: Transferase]]
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