8cat: Difference between revisions
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New page: left|200px<br /> <applet load="8cat" size="450" color="white" frame="true" align="right" spinBox="true" caption="8cat, resolution 2.5Å" /> '''THE NADPH BINDING SI... |
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[[Image:8cat.gif|left|200px]]<br /> | [[Image:8cat.gif|left|200px]]<br /><applet load="8cat" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="8cat" size=" | |||
caption="8cat, resolution 2.5Å" /> | caption="8cat, resolution 2.5Å" /> | ||
'''THE NADPH BINDING SITE ON BEEF LIVER CATALASE'''<br /> | '''THE NADPH BINDING SITE ON BEEF LIVER CATALASE'''<br /> | ||
==Overview== | ==Overview== | ||
Beef liver and human erythrocyte catalases (EC 1.11.1.6) bind NADP | Beef liver and human erythrocyte catalases (EC 1.11.1.6) bind NADP tenaciously [Kirkman, H. N. & Gaetani, G. F. (1984) Proc. Natl. Acad. Sci. USA 81, 4343-4348]. The position of NADP on beef liver catalase corresponds to the carboxyl-terminal polypeptide hinge in Penicillium vitale fungal catalase, which connects the common catalase structure to the additional flavodoxin-like domain. In contrast to nearly all other known structures of protein-bound NADP, NAD, and FAD, the NADP molecule of beef liver catalase is folded into a right-handed helix and bound, in part, in the vicinity of the carboxyl end of two alpha-helices. A water molecule (W7) occupies a pseudosubstrate site close to the C4 position of the nicotinamide and is hydrogen bonded to His-304. Although the NADP and heme groups approach each other to within 13.7 A, there is no direct interaction. The function of the NADP remains a mystery. | ||
==About this Structure== | ==About this Structure== | ||
8CAT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with HEM and NDP as [http://en.wikipedia.org/wiki/ligands ligands]. This structure | 8CAT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=NDP:'>NDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entries 3CAT and 1CAT. The following page contains interesting information on the relation of 8CAT with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb57_1.html Catalase]]. Active as [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CAT OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Catalase]] | [[Category: Catalase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: /III, T | [[Category: /III, T J.Reid.]] | ||
[[Category: Fita, I.]] | [[Category: Fita, I.]] | ||
[[Category: Murthy, M | [[Category: Murthy, M R.N.]] | ||
[[Category: Rossmann, M | [[Category: Rossmann, M G.]] | ||
[[Category: Sicignano, A.]] | [[Category: Sicignano, A.]] | ||
[[Category: Tanaka, N.]] | [[Category: Tanaka, N.]] | ||
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[[Category: oxidoreductase (h2o2 acceptor)]] | [[Category: oxidoreductase (h2o2 acceptor)]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:17:50 2008'' | ||
