Sandbox Reserved 918: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 42: | Line 42: | ||
===Active Site=== | ===Active Site=== | ||
These substrates, along with many others, are cleaved by DPP IV at its active site containing a [http://en.wikipedia.org/wiki/Catalytic_triad catalytic triad] composed of <scene name='57/573132/1x70_catalytictriad/1'>Ser630, His740, and Asp708</scene>. | These substrates, along with many others, are cleaved by DPP IV at its active site containing a [http://en.wikipedia.org/wiki/Catalytic_triad catalytic triad] composed of <scene name='57/573132/1x70_catalytictriad/1'>Ser630, His740, and Asp708</scene>. The substrate shown is a DPP IV inhibitorThis Serine-Histadine-Asparatate motif, best known in the enzyme [http://en.wikipedia.org/wiki/Chymotrypsin chymotrypsin], uses acid-base chemistry to facilitate the binding, cleaving, and release of the given substrate. The mechanism of the reaction is as follows: | ||
<div style="text-align: left;"> | <div style="text-align: left;"> | ||
| Line 53: | Line 53: | ||
# The negative oxygen on serine readily accepts the hydrogen from histadine and in doing so regenerates the active site of the enzyme. | # The negative oxygen on serine readily accepts the hydrogen from histadine and in doing so regenerates the active site of the enzyme. | ||
</div> | </div> | ||
In addition to the glutamates holding the substrate in close proximity, and the catalytic triad using acid base chemistry to cleave the peptide bond, there is a tyrosine, <scene name='57/573132/1x70_activesitetyr/2'>Tyr547</scene>, which is depicted in orange and notably only 4.08 [http://en.wikipedia.org/wiki/Angstrom angstroms] away from the substrate, [http://en.wikipedia.org/wiki/Sitagliptin Sitagliptin]. | |||
</StructureSection> | </StructureSection> | ||
