User:Alexander Rudecki/Sandbox 1: Difference between revisions
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==Structure== | ==Structure== | ||
<Structure load='4F9U' size='350' frame='true' align='right' caption='A 3D ribbon model of DromeQC as determined by X-ray crystallography' scene='Insert optional scene name here'/> | <Structure load='4F9U' size='350' frame='true' align='right' caption='A 3D ribbon model of DromeQC as determined by X-ray crystallography' scene='Insert optional scene name here'/> | ||
===Visual Walkthrough=== | |||
DromeQC is a <scene name='58/580851/Two_chains/2'>homodimer</scene> consisting of an <span style="font-size:150%"><font color='blue'>A Chain</font></span> and a <span style="font-size:150%"><font color='limegreen'>B Chain</font></span>. This homodimer can also be viewed in <scene name='58/580851/Trace/1'>trace</scene> and <scene name='58/580851/Space_fill/1'>space filling</scene> representations. The backbone of DromeQC can be easily seen as an <scene name='58/580851/N_to_c_rainbow/1'>N to C rainbow</scene>. Here the polypeptide progression is depicted by the following template: | DromeQC is a <scene name='58/580851/Two_chains/2'>homodimer</scene> consisting of an <span style="font-size:150%"><font color='blue'>A Chain</font></span> and a <span style="font-size:150%"><font color='limegreen'>B Chain</font></span>. This homodimer can also be viewed in <scene name='58/580851/Trace/1'>trace</scene> and <scene name='58/580851/Space_fill/1'>space filling</scene> representations. The backbone of DromeQC can be easily seen as an <scene name='58/580851/N_to_c_rainbow/1'>N to C rainbow</scene>. Here the polypeptide progression is depicted by the following template: | ||
{{Template:ColorKey_Amino2CarboxyRainbow}} | {{Template:ColorKey_Amino2CarboxyRainbow}} | ||
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===Topology and Overall Structure=== | ===Topology and Overall Structure=== | ||
DromeQC is made up of 2 identical, independent monomers that come together to form an asymmetric homodimer (Figure 1). The subunits are connected via 4 hydrogen bonds (Chain 1→Chain 2: ARG35 NH2→GLU64 OE2, ARG43 NH2→ASN71 O, ARG43 NH2→PHE75 O, ARG43 NH1→PHE75 O) and surface complementarity. The subunits exhibit a globular α/β-hydrolase fold, characterized by a central twisted β-sheet motif consisting of 5 parallel strands (β1 and β3-β6) and an antiparallel β2 strand (Figure 2). This β-center is flanked by 9 surrounding α-helices; 2 fill the concave face (α5, α7), 7 fill the convex face (α1- α5, α8, α9) with one helix at the edge (α6) of each monomer. DromeQC is glycosylated (with up to 7 carbohydrate moieties) at the N42 position. These polysaccharide tags increased solubility of DromeQC, and appear to have no affect of protein activity. <ref name="main"/>. | As shown above, DromeQC is made up of 2 identical, independent monomers that come together to form an asymmetric homodimer (Figure 1). The subunits are connected via 4 hydrogen bonds (Chain 1→Chain 2: ARG35 NH2→GLU64 OE2, ARG43 NH2→ASN71 O, ARG43 NH2→PHE75 O, ARG43 NH1→PHE75 O) and surface complementarity. The subunits exhibit a globular α/β-hydrolase fold, characterized by a central twisted β-sheet motif consisting of 5 parallel strands (β1 and β3-β6) and an antiparallel β2 strand (Figure 2). This β-center is flanked by 9 surrounding α-helices; 2 fill the concave face (α5, α7), 7 fill the convex face (α1- α5, α8, α9) with one helix at the edge (α6) of each monomer. DromeQC is glycosylated (with up to 7 carbohydrate moieties) at the N42 position. These polysaccharide tags increased solubility of DromeQC, and appear to have no affect of protein activity. <ref name="main"/>. | ||
