Sandbox reserved 919: Difference between revisions
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[[Image:Proteopediapic.png|300 px|left|thumb|Hormone-Sensitive Lipase Complex with [http://proteopedia.org/wiki/index.php/3h17 3h17]]] | [[Image:Proteopediapic.png|300 px|left|thumb|Hormone-Sensitive Lipase Complex with [http://proteopedia.org/wiki/index.php/3h17 3h17]]] | ||
Hormone-sensitive lipase can be inhibited by phenylmethylsufonyl flouride ([http://en.wikipedia.org/wiki/PMSF PMSF]) entering into the <scene name='58/580296/Meshligand/2'>active site</scene>. PMSF inhibits enzymes by binding to the | Hormone-sensitive lipase can be inhibited by phenylmethylsufonyl flouride ([http://en.wikipedia.org/wiki/PMSF PMSF]) entering into the <scene name='58/580296/Meshligand/2'>active site</scene>. The experiments performed to test this inhibition used different strains of bacteria, which had a different order of residues but the same catalytic effect. This experiment tested the Ser144 residue rather than the Ser157 residue seen in other HSL proteins. PMSF inhibits enzymes by binding to the Ser144 residue of the [http://proteopedia.org/wiki/index.php/Serine_Proteases serine protease] active sites so that the normal catalytic activity cannot be carried out. This inhibitor will only bind to the active site Ser144 because of its participation in the charge relay of the <scene name='58/580296/Meshligandinteraction/1'>catalytic triad</scene>. This hyper activity allows the sulfonyl group of PMSF to <scene name='58/580296/Inhibitorinteraction/4'>covalently bond</scene> to the Ser144 residue to disrupt its activity. Because of this Ser144 residue specificity, PMSF does not inhibit all kinds of lipases, just those dependent on Ser144 residues in the active site. PMSF is highly degradable in aqueous solutions so it does not inhibit for very long periods of time in its natural environment. PMSF binding induces only a minor conformational change from the native protein.<ref name="Kanwar">PMID:23923547</ref> | ||
Inhibition or decreased activity of hormone-sensitive lipases can possibly lead to disorders such as atherosclerosis, obesity, and type 2 diabetes. Inability to break down fatty acid molecules due to inactive hormone-sensitive lipases has been reported in many cases of obesity and type 2 diabetes. Increased inhibition of HSL by PMSF could, in theory, also be a possible cause of these diseases.<ref name="Kraemer">PMID: 12364542 </ref> | Inhibition or decreased activity of hormone-sensitive lipases can possibly lead to disorders such as atherosclerosis, obesity, and type 2 diabetes. Inability to break down fatty acid molecules due to inactive hormone-sensitive lipases has been reported in many cases of obesity and type 2 diabetes. Increased inhibition of HSL by PMSF could, in theory, also be a possible cause of these diseases.<ref name="Kraemer">PMID: 12364542 </ref> | ||
