Sandbox Reserved 191: Difference between revisions
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==Catalytic Triad== | |||
PPT-1's structure creates an external hydrophobic groove that binds the palmitate acid in-between carbon 4 and 5. The acid binds in a gauche conformations [https://en.wikipedia.org/wiki/Gauche_effect] creating a kink in the acid chain. This bending could suggest that PPT-1 was originally designed to react with unsaturated fatty acid with cis-double bonds. The catalytic <scene name='43/436866/Triad_w_zoom/2'>triad</scene> is composed of Serine 115, Aspartate 233, and Histidine 289. The Serine is "deprotonated" by the Histidine and attacks the carbonyl carbon of the palmitic acid. The negative charge is pushed onto the oxygen and is possibly stabilized by a water molecule. The tetrahedral intermediate collapses and kicks the palmatic acid off of the cysteine residue<ref name="mutations" />. | PPT-1's structure creates an external hydrophobic groove that binds the palmitate acid in-between carbon 4 and 5. The acid binds in a gauche conformations [https://en.wikipedia.org/wiki/Gauche_effect] creating a kink in the acid chain. This bending could suggest that PPT-1 was originally designed to react with unsaturated fatty acid with cis-double bonds. The catalytic <scene name='43/436866/Triad_w_zoom/2'>triad</scene> is composed of Serine 115, Aspartate 233, and Histidine 289. The Serine is "deprotonated" by the Histidine and attacks the carbonyl carbon of the palmitic acid. The negative charge is pushed onto the oxygen and is possibly stabilized by a water molecule. The tetrahedral intermediate collapses and kicks the palmatic acid off of the cysteine residue<ref name="mutations" />. | ||
[[Image:Simple RXN.png|400px|right|thumb|The basic reaction]] | [[Image:Simple RXN.png|400px|right|thumb|The basic reaction]] | ||
==Inhibitors of PPT-1== | ==Inhibitors of PPT-1== | ||
