Proteinase: Difference between revisions
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Michal Harel (talk | contribs) New page: <StructureSection load='2duj' size='340' side='right' caption='E. coli proteinase K complex with lactoferrin peptide and Ca+2 (PDB code 2duj)' scene=''> '''Proteinase''' (PRO) are enz... |
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<StructureSection load='2duj' size='340' side='right' caption='E. coli proteinase K complex with lactoferrin peptide and Ca+2 (PDB code [[2duj]])' scene=''> | <StructureSection load='2duj' size='340' side='right' caption='E. coli proteinase K complex with lactoferrin peptide and Ca+2 (PDB code [[2duj]])' scene=''> | ||
'''Proteinase''' (PRO) are enzymes which hydrolyze peptide bonds. They are classified by the amino acid site of their cleavage or by the pH at which they are active. PRO B is a serine protease. For more details on PRO B see [[Streptomyces griseus proteinase B]]. PRO A is a carboxylproteinase. | '''Proteinase''' (PRO) are enzymes which hydrolyze peptide bonds. They are classified by the amino acid site of their cleavage or by the pH at which they are active. PRO B is a serine protease. For more details on PRO B see [[Streptomyces griseus proteinase B]]. PRO A is a carboxylproteinase. PRO K is a serine protease which cleaves proteins preferentially after hydrophobic residues. Calcium ions contribute to the stability of the enzyme. PRO is active over a wide pH range and is used in molecular biology to inactivate nucleases from preparations of DNA or RNA. PRO is used in the partial proteolysis of lactoferrin into its N- and C-lobe. The two lobes of lactoferrin have different antimicrobial and antifungal properties. PRO K can digest hair (keratin). | ||
==3D structures of proteinase== | ==3D structures of proteinase== | ||
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'''PRO A''' | '''PRO A''' | ||
2sga – SgPRO – ''Streptomyces griseus'' | [[2sga]] – SgPRO – ''Streptomyces griseus''<br /> | ||
2jxr, 1fmu, 1fmx – yPRO - yeast | [[2jxr]], [[1fmu]], [[1fmx]] – yPRO - yeast<br /> | ||
1sgc - SgPRO + chymostatin A | [[1sgc]] - SgPRO + chymostatin A<br /> | ||
3sga, 4sga, 5sga - SgPRO + polypeptide inhibitor | [[3sga]], [[4sga]], [[5sga]] - SgPRO + polypeptide inhibitor<br /> | ||
1dp5, 1dpj, 1g0v - yPRO + polypeptide inhibitor IA3 | [[1dp5]], [[1dpj]], [[1g0v]] - yPRO + polypeptide inhibitor IA3<br /> | ||
1fq5 - yPRO + inhibitor | [[1fq5]] - yPRO + inhibitor | ||
'''PRO B''' | '''PRO B''' | ||
[[sgb]] – SgPRO + turkey ovomucoid inhibitor<br /> | |||
1sgp, 1sgq, 1sgr, 1cso, 1ct0, 1ct2, 1ct4, 1ds2, 2sgp, 2nu3, 2nu4 – SgPRO + turkey ovomucoid inhibitor (mutant) | [[1sgp]], [[1sgq]], [[1sgr]], [[1cso]], [[1ct0]], [[1ct2]], [[1ct4]], [[1ds2]], [[2sgp]], [[2nu3]], [[2nu4]] – SgPRO + turkey ovomucoid inhibitor (mutant)<br /> | ||
4sgb - SgPRO + potato inhibitor | [[4sgb]] - SgPRO + potato inhibitor | ||
'''PRO K''' | '''PRO K''' | ||
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[[1oyo]] – EaPRO + Ca + melanin monomer <br /> | [[1oyo]] – EaPRO + Ca + melanin monomer <br /> | ||
[[3dyb]] – EaPRO + Ca + digalacturonic acid <br /> | [[3dyb]] – EaPRO + Ca + digalacturonic acid <br /> | ||
'''PRO 3C''' | '''PRO 3C''' | ||
1qa7 – PRO – Hepatitis virus | [[1qa7]] – PRO – Hepatitis virus<br /> | ||
2vb0 - PRO – Coxsakievirus | [[2vb0]] - PRO – Coxsakievirus | ||
'''H2-PRO''' | '''H2-PRO''' | ||
1wni – PRO – ''Trimeresurus flavoviridis'' | [[1wni]] – PRO – ''Trimeresurus flavoviridis'' | ||
'''Aspartic PRO''' | '''Aspartic PRO''' | ||
2asi – PRO – ''Rhizomucor miehei'' | [[2asi]] – PRO – ''Rhizomucor miehei''<br /> | ||
1zap – CaPRO – ''Candida albicans'' | [[1zap]] – CaPRO – ''Candida albicans''<br /> | ||
1izd - AoPRO – ''Aspergillus oryzae'' | [[1izd]] - AoPRO – ''Aspergillus oryzae''<br /> | ||
1eag – CaPRO + inhibitor | [[1eag]] – CaPRO + inhibitor <br /> | ||
1fq4 - yPRO + inhibitor | [[1fq4]] - yPRO + inhibitor<br /> | ||
1j71 - PRO + polypeptide inhibitor – ''Candida tropicalis'' | [[1j71]] - PRO + polypeptide inhibitor – ''Candida tropicalis''<br /> | ||
1ize - AoPRO + polypeptide-statine inhibitor | [[1ize]] - AoPRO + polypeptide-statine inhibitor<br /> | ||
1wkr - PRO + polypeptide-statine inhibitor – ''Irpex lacteus'' | [[1wkr]] - PRO + polypeptide-statine inhibitor – ''Irpex lacteus'' | ||
'''Cysteine PRO''' | '''Cysteine PRO''' | ||
2hrv – PRO 2A – human rhinovirus | [[2hrv]] – PRO 2A – human rhinovirus | ||
'''Serine PRO''' | '''Serine PRO''' | ||
1s2n, 1sh7 – PRO – Vibrio | [[1s2n]], [[1sh7]] – PRO – ''Vibrio''<br /> | ||
3s9a, 3s9b – RvPRO – Siamese Russell’s viper | [[3s9a]], [[3s9b]] – RvPRO – Siamese Russell’s viper<br /> | ||
3s9c, 3sbk – RvPRO + human factor V polypeptide | [[3s9c]], [[3sbk]] – RvPRO + human factor V polypeptide<br /> | ||
1ga1, 1ga4, 1ga6, 1nlu – PsPRO + iodotyrostatin fragment – ''Pseudomonas'' | [[1ga1]], [[1ga4]], [[1ga6]], [[1nlu]] – PsPRO + iodotyrostatin fragment – ''Pseudomonas''<br /> | ||
1kdv, 1kdy, 1kdz, 1ke1, 1ke2 - PsPRO + polypeptide inhibitor | [[1kdv]], [[1kdy]], [[1kdz]], [[1ke1]], [[1ke2]] - PsPRO + polypeptide inhibitor | ||
