2ghw: Difference between revisions
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{{STRUCTURE_2ghw| PDB=2ghw | SCENE= }} | {{STRUCTURE_2ghw| PDB=2ghw | SCENE= }} | ||
===Crystal structure of SARS spike protein receptor binding domain in complex with a neutralizing antibody, 80R=== | ===Crystal structure of SARS spike protein receptor binding domain in complex with a neutralizing antibody, 80R=== | ||
{{ABSTRACT_PUBMED_16954221}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. | |||
==About this Structure== | |||
[[2ghw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHW OCA]. | |||
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== | ==See Also== | ||
*[[Monoclonal Antibody|Monoclonal Antibody]] | |||
==Reference== | ==Reference== | ||
<ref group="xtra">PMID: | <ref group="xtra">PMID:016954221</ref><references group="xtra"/><references/> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Sars coronavirus]] | [[Category: Sars coronavirus]] | ||
| Line 38: | Line 28: | ||
[[Category: S protein]] | [[Category: S protein]] | ||
[[Category: Sar]] | [[Category: Sar]] | ||
[[Category: Virus | [[Category: Virus-viral protein-antibiotic complex]] | ||
Revision as of 08:44, 23 April 2014
Crystal structure of SARS spike protein receptor binding domain in complex with a neutralizing antibody, 80R
Template:ABSTRACT PUBMED 16954221
Function
[SPIKE_CVHSA] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
About this Structure
2ghw is a 4 chain structure with sequence from Homo sapiens and Sars coronavirus. Full crystallographic information is available from OCA.
See Also
Reference
- Hwang WC, Lin Y, Santelli E, Sui J, Jaroszewski L, Stec B, Farzan M, Marasco WA, Liddington RC. Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R. J Biol Chem. 2006 Nov 10;281(45):34610-6. Epub 2006 Sep 5. PMID:16954221 doi:10.1074/jbc.M603275200
