4o8u

Structure of PF2046

spinqualitylabelsall models 4o8u, resolution 2.34Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4o8u is a 6 chain structure. Full crystallographic information is available from OCA. Activity: Glucokinase, with EC number 2.7.1.2 Publication Abstract from PubMed Sometimes crystals cannot diffract X-rays beyond 3.0 A resolution due to the intrinsic flexibility associated with the protein. Low resolution diffraction data not only pose a challenge to structure determination, but also hamper interpretation of mechanistic details. Crystals of a 25.6 kDa non-Pfam, hypothetical protein, PF2046, diffracted X-rays to 3.38 A resolution. A combination of Se-Met derived heavy atom positions with multiple cycles of B-factor sharpening, multi-crystal averaging, restrained refinement followed by manual inspection of electron density and model building resulted in a final model with a R value of 23.5 (R(free)= 24.7). The asymmetric unit was large and consisted of six molecules arranged as a homodimer of trimers. Analysis of the structure revealed the presence of a RNA binding domain suggesting a role for PF2046 in the processing of nucleic acids. Crystal structure of a novel non-Pfam protein PF2046 solved using low resolution B-factor sharpening and multi-crystal averaging methods.,Su J, Li Y, Shaw N, Zhou W, Zhang M, Xu H, Wang BC, Liu ZJ Protein Cell. 2010 May;1(5):453-8. doi: 10.1007/s13238-010-0045-7. Epub 2010 Jun , 4. PMID:21203960 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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