4ou0: Difference between revisions
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''' | ==Crystal Structure of RPA32C== | ||
<StructureSection load='4ou0' size='340' side='right' caption='[[4ou0]], [[Resolution|resolution]] 1.40Å' scene=''> | |||
== Structural highlights == | |||
[[4ou0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU0 OCA]. <br> | |||
<b>Related:</b> [[1dpu|1dpu]]<br> | |||
<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br> | |||
== Publication Abstract from PubMed == | |||
SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A-like1 (SMARCAL1) is a recently identified DNA damage response protein involved in remodeling stalled replication forks. The eukaryotic single-strand (ss) DNA-binding protein Replication Protein A (RPA) recruits SMARCAL1 to stalled forks in vivo and facilitates regression of forks containing leading strand gaps. Both activities are mediated by a direct interaction between an RPA-binding motif (RBM) at the N-terminus of SMARCAL1 and the C-terminal winged-helix domain of the RPA 32-kDa subunit (RPA32C). Here we report a biophysical and structural characterization of the SMARCAL1-RPA interaction. Isothermal titration calorimetry and CD spectroscopy revealed that RPA32C binds the SMARCAL1-RBM with a Kd of 3 muM and induces a disorder-to-helix transition. The crystal structure of RPA32C was refined to 1.4 A resolution and the SMARCAL1-RBM binding site was mapped on the structure on the basis of NMR chemical shift perturbations. Conservation of the interaction surface to other RBM-containing proteins enabled construction of a model for the RPA32C/SMARCAL1-RBM complex. The implications of our results are discussed with respect to the recruitment of SMARCAL1 and other DNA damage response and repair proteins to stalled replication forks. | |||
Structural analysis of RPA recruitment of the DNA damage response protein SMARCAL1.,Feldkamp MD, Mason AC, Eichman BF, Chazin WJ Biochemistry. 2014 Apr 15. PMID:24730652<ref>PMID:24730652</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Chazin, W J.]] | |||
[[Category: Eichman, B F]] | |||
[[Category: Feldkamp, M D.]] | |||
[[Category: Mason, A C.]] | |||
[[Category: Dna binding protein]] | |||
[[Category: Protein-protein interaction]] | |||
[[Category: S-methyl-thio-cysteine]] | |||
[[Category: Winged-helix turn helix]] | |||
