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[[Image:2ljx.png|left|200px]]
==Structure of the monomeric N-terminal domain of HPV16 E6 oncoprotein==
<StructureSection load='2ljx' size='340' side='right' caption='[[2ljx]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
== Structural highlights ==
[[2ljx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_papillomavirus Human papillomavirus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LJX OCA]. <br>
<b>Related:</b> [[2ljy|2ljy]], [[2ljz|2ljz]]<br>
<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
== Publication Abstract from PubMed ==
The viral oncoprotein E6 is an essential factor for cervical cancers induced by "high-risk" mucosal HPV. Among other oncogenic activities, E6 recruits the ubiquitin ligase E6AP to promote the ubiquitination and subsequent proteasomal degradation of p53. E6 is prone to self-association, which long precluded its structural analysis. Here we found that E6 specifically dimerizes through its N-terminal domain and that disruption of the dimer interface strongly increases E6 solubility. This allowed us to raise structural data covering the entire HPV16 E6 protein, including the high-resolution NMR structures of the two zinc-binding domains of E6 and a robust data-driven model structure of the N-terminal domain homodimer. Interestingly, homodimer interface mutations that disrupt E6 self-association also inactivate E6-mediated p53 degradation. These data suggest that E6 needs to self-associate via its N-terminal domain to promote the polyubiquitination of p53 by E6AP.


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Solution Structure Analysis of the HPV16 E6 Oncoprotein Reveals a Self-Association Mechanism Required for E6-Mediated Degradation of p53.,Zanier K, Ould M'hamed Ould Sidi A, Boulade-Ladame C, Rybin V, Chappelle A, Atkinson A, Kieffer B, Trave G Structure. 2012 Apr 4;20(4):604-17. Epub 2012 Apr 3. PMID:22483108<ref>PMID:22483108</ref>
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{{STRUCTURE_2ljx|  PDB=2ljx  |  SCENE=  }}


===Structure of the monomeric N-terminal domain of HPV16 E6 oncoprotein===
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
 
== References ==
 
<references/>
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{{ABSTRACT_PUBMED_22483108}}
 
==About this Structure==
[[2ljx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_papillomavirus Human papillomavirus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LJX OCA].
 
==Reference==
<ref group="xtra">PMID:022483108</ref><references group="xtra"/>
[[Category: Human papillomavirus]]
[[Category: Human papillomavirus]]
[[Category: Atkinson, A.]]
[[Category: Atkinson, A.]]
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