2kxn: Difference between revisions

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[[Image:2kxn.png|left|200px]]
==NMR structure of human Tra2beta1 RRM in complex with AAGAAC RNA==
<StructureSection load='2kxn' size='340' side='right' caption='[[2kxn]], [[NMR_Ensembles_of_Models | 12 NMR models]]' scene=''>
== Structural highlights ==
[[2kxn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KXN OCA]. <br>
<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
== Publication Abstract from PubMed ==
Tra2-beta1 is a unique splicing factor as its single RNA recognition motif (RRM) is located between two RS (arginine-serine) domains. To understand how this protein recognizes its RNA target, we solved the structure of Tra2-beta1 RRM in complex with RNA. The central 5'-AGAA-3' motif is specifically recognized by residues from the beta-sheet of the RRM and by residues from both extremities flanking the RRM. The structure suggests that RNA binding by Tra2-beta1 induces positioning of the two RS domains relative to one another. By testing the effect of Tra2-beta1 and RNA mutations on the splicing of SMN2 exon 7, we validated the importance of the RNA-protein contacts observed in the structure for the function of Tra2-beta1 and determined the functional sequence of Tra2-beta1 in SMN2 exon 7. Finally, we propose a model for the assembly of multiple RNA binding proteins on this exon.


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Molecular basis of purine-rich RNA recognition by the human SR-like protein Tra2-beta1.,Clery A, Jayne S, Benderska N, Dominguez C, Stamm S, Allain FH Nat Struct Mol Biol. 2011 Apr;18(4):443-50. Epub 2011 Mar 13. PMID:21399644<ref>PMID:21399644</ref>
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{{STRUCTURE_2kxn|  PDB=2kxn  |  SCENE=  }}


===NMR structure of human Tra2beta1 RRM in complex with AAGAAC RNA===
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
 
== References ==
 
<references/>
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==About this Structure==
[[2kxn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KXN OCA].
 
==Reference==
<ref group="xtra">PMID:021399644</ref><references group="xtra"/>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Allain, F H.T.]]
[[Category: Allain, F H.T.]]
Retrieved from "https://proteopedia.org/2kxn"